FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11440
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT617253.95104895104895No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA156213.653846153846153No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC2512.194055944055944No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2352.054195804195804Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC2332.0367132867132867No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1931.6870629370629369No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT1070.9353146853146853No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC710.6206293706293706No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT660.576923076923077No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA630.5506993006993007Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA480.4195804195804196No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT430.3758741258741259No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA430.3758741258741259No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA410.3583916083916084No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA400.34965034965034963No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC370.32342657342657344Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC330.2884615384615385Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA320.27972027972027974No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT310.27097902097902093No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA300.26223776223776224Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT300.26223776223776224No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCTCTA270.23601398601398602No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT260.22727272727272727No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCTCTATG260.22727272727272727No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA260.22727272727272727No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA260.22727272727272727No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCAGGGCTTCACTTACTCT240.2097902097902098No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.19230769230769232No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.19230769230769232No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.19230769230769232No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT210.18356643356643357No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA190.1660839160839161No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCTCTAT190.1660839160839161No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT190.1660839160839161No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT170.1486013986013986No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT160.13986013986013987No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT160.13986013986013987No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC160.13986013986013987No Hit
CAACCAAAAGAGAAGGAGGCACGATTCCATCAAAGAGAGACAGCACCCTC150.13111888111888112No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT150.13111888111888112No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA150.13111888111888112No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT140.12237762237762238No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA130.11363636363636363No Hit
TCCTATCAGTGGTCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT130.11363636363636363No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT130.11363636363636363No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT130.11363636363636363No Hit
TCCTATCAGTGGCCACAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT120.1048951048951049No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)