FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12076
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA393432.57701225571381No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA249420.652533951639615No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC4914.0659158661808545No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA3993.3040741967538922No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA3492.89002981119576No Hit
GTGCTGTCTCTCTTTGATGGACGAAGAGGAAACCAGGATGTGCAGCCAAG2702.235839682013912No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG1661.3746273600529977No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1581.3083802583636965No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG1491.2338522689632327No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1361.1262007287181186No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1271.0516727393176548Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC860.7121563431599868No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC700.5796621397813845No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG660.546538588936734No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG600.4968532626697582No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA540.44716793640278235No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGTTACGC490.4057634978469692No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC450.37263994700231867Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA440.36435905929115603No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA420.34779728386883074No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA400.33123550844650546No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT400.33123550844650546Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTTACGCAG390.32295462073534287No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG370.3063928453130175No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG360.29811195760185494No Hit
GTGCTGTCTCTCTTTGATGGAGCAACTCTGCGTTGATACCACTGCTTCCC350.28983106989069224Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA340.28155018217952965No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC340.28155018217952965No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCCTCC300.2484266313348791No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA280.23186485591255385No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT250.20702219277906592No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG240.1987413050679033No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCCT230.19046041735674063No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.17389864193441537No Hit
GTGCTGTCTCTCTTTGATGGAGAGTGGGTACTCGTTAACGAGATCTGGTT210.17389864193441537No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT210.17389864193441537No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.15733686651209008No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG180.14905597880092747No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG170.14077509108976483No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAT160.13249420337860218No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA160.13249420337860218No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCCTCCTT160.13249420337860218No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG150.12421331566743955No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA150.12421331566743955No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA150.12421331566743955No Hit
GTGCTGTCTCTCTTTGATGGAGCGGTGCTGTCTCTCTTTGATGGAGCGGT150.12421331566743955No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT140.11593242795627692Clontech SMART CDS Primer II A (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC140.11593242795627692No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA140.11593242795627692No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGTAGGGCTCCTGGTGCTGA130.10765154024511428No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC130.10765154024511428No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGACTCCTGGTGCTGA130.10765154024511428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)