FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5138
Total Bases744.8 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA358369.73530556636824No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1402.7247956403269753No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1182.2966134682755937No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT981.9073569482288828No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA681.3234721681588166No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA470.9147528221097703No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA470.9147528221097703No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA420.8174386920980926No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA410.7979758660957572No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC380.7395873880887505No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA350.6811989100817438No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA320.6228104320747372No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC240.46710782405605294No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT230.44764499805371744No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.42818217205138187No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT200.3892565200467108No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA190.36979369404437523No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA180.3503308680420397No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA140.2724795640326975No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT140.2724795640326975No Hit
GGGCAAAGGGTGAGGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA120.23355391202802647No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA120.23355391202802647No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA100.1946282600233554Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAGTGAAAGGAGGCA100.1946282600233554No Hit
GGGCAAAGGGTGAAGAGAAAGAGGACAAATGAACAAAATGAAAGGAGGCA100.1946282600233554No Hit
GGGCAAAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG100.1946282600233554Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGGGGCA90.17516543402101986No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGACA80.1557026080186843No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAGCAAAATGAAAGGAGGCA80.1557026080186843No Hit
GGGCAAAGGGTGAAAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA80.1557026080186843No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA80.1557026080186843No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAGAGGAGGCA80.1557026080186843No Hit
GGGCAAAGGGTGAAGAAAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA70.13623978201634876No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGGAAGGAGGCA70.13623978201634876No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAGTGAACAAAATGAAAGGAGGCA70.13623978201634876No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGGACAAAATGAAAGGAGGCA70.13623978201634876No Hit
GGGCAAAGGGTGAAGAGAAAGGGGGCAAATGAACAAAATGAAAGGAGGCA60.11677695601401324No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGTA60.11677695601401324No Hit
GGGCAAAGGGTGAAGAGAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA60.11677695601401324No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGAA60.11677695601401324No Hit
GGGCAAAGGGTGAAGGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA60.11677695601401324No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)