FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42039
Total Bases6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA1633638.85915459454316No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1073125.526297009919364No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA493611.741478151240514No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG4631.1013582625657128No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC4611.0966007754703966No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG4140.9847998287304646No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC2560.6089583482004804No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1550.3687052498870097No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA1460.3472965579580865No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1460.3472965579580865Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG1250.29734294345726586No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGATGCTGAAGGA1200.28544922571897524No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG1190.2830704821713171No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA1130.26879802088536836No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1100.261661790242394No Hit
GTGCTGTCTCTCTTTATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC1090.25928304669473584No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC1090.25928304669473584No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA1000.23787435476581273No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG990.23549561121815457No Hit
TGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC890.21170817574157327No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA880.2093294321939152No Hit
GTGCTGTCTCTCTTTGAGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC840.19981445800328268No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG820.19505697090796642No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA780.1855419967173339No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC760.18078450962201764No Hit
GTGCTGTCTCTCTTTGTGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC750.1784057660743595No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT670.15937581769309453No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA640.1522395870501201No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG630.149860843502462No Hit
GTGCTGTCTCTCTTGGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA630.149860843502462No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA620.14748209995480388No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGACTCCTGGTGCTGAAGGA610.14510335640714575No Hit
GTGCTGTCTCCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC580.13796712576417136No Hit
GTGCTGTCTTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC580.13796712576417136No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA570.13558838221651323No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT560.1332096386688551No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC560.1332096386688551No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG560.1332096386688551No Hit
CTCCAAGTAACGGTGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG550.130830895121197No Hit
GTGCTGTCCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC510.12131592093056448No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT490.11655843383524822No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG460.10942220319227385No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGG450.10704345964461572No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA440.1046647160969576No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)