FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5263
Total Bases762.6 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA155529.545886376591294No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC3676.973209196275889No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA2695.111153334600038No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1392.6410792323769714No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1302.470074102223067No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1172.22306669200076Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG981.8620558616758505No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC861.6340490214706442No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG851.6150484514535435No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA811.5390461713851415No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT490.9310279308379252No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC430.8170245107353221No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT410.779023370701121Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA370.703021090632719No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC370.703021090632719Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG360.6840205206156185No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG360.6840205206156185No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTTACGCAG340.6460193805814174No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA300.5700171005130154No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA280.5320159604788144No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA270.5130153904617138No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA260.49401482044461337No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC260.49401482044461337No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG250.47501425042751283No Hit
CTCCAAGTAACGGTGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTCG230.4370131103933118No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA230.4370131103933118No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA230.4370131103933118No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT210.39901197035911073No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA180.34201026030780923No Hit
GCAAAAGTTCTCAGGGATGTCCATGCATTCGTTTTTCTTTAAAACTTGAT180.34201026030780923No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGTTACGC170.3230096902907087No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCCTCC170.3230096902907087No Hit
CTCCAAGTAACGGTGCGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTCG160.3040091202736082No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC140.2660079802394072No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAT130.24700741022230668No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC130.24700741022230668No Hit
GTGCTGTCTCTCTTTGATGGATGCTGTCTCTCTTTGATGGGTGCTGTCTC130.24700741022230668No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA130.24700741022230668No Hit
GTGCTGTCTCTCTTTGATGGAATCCTGGGTAATAAAGCATTTGGCTGACG120.22800684020520615No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCCTCCTT110.20900627018810564No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT100.19000570017100513No Hit
GTGCTGTCTCTCTTTGATGGAGGGTGCTGTCTCTCTTTGATGGAGGGTGC100.19000570017100513No Hit
GCAAAAGTTCTCAGGGATGTCCATCACTTTCTGAACTGTCTGTGTGCTCG100.19000570017100513No Hit
GTGCTGTCTCTCTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTATC90.17100513015390462No Hit
GTGCTGTCTCTCTTTGATGGTCCTCACTTTGCTGAACTTTTGCCTCCCAT90.17100513015390462No Hit
GTGCTGTCTCTCTTTGATGGAATCTGCGTTGATACCACTGCTTCCCATGT80.1520045601368041Clontech Universal Primer Mix Long (95% over 23bp)
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA80.1520045601368041No Hit
GCAAAAGTTCTCAGGGATGTCCGTGCCTCCTTCTCTTTTGGTTGTTACGC80.1520045601368041No Hit
GTGCTGTCTCTCTTTGATGGACTGTACCAGGTGCGCTCCTATCTTGGGCC80.1520045601368041No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA80.1520045601368041No Hit
CTCCAAGTAACGGTGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG80.1520045601368041No Hit
GCAAAAGTTCTCAGGGATGTGTCATCCCCAAGCAGCGGTCATCCCCAAGC80.1520045601368041No Hit
CTCCAAGTAACGGTGCTGTCTGTTGGCTGGTGCAGGGCTCCTGGTGCTGA80.1520045601368041No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC80.1520045601368041No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTA80.1520045601368041No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA70.1330039901197036No Hit
GTGCTGTCTCTCTTTGATGTTACGCAGACCAAGTCTCTGCTACCGTATCC70.1330039901197036No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCCT70.1330039901197036No Hit
CTCCAAGTAACGGTGCTGAGAAAGCAGAAATGAGTTCAGCAAAGTGAGGA70.1330039901197036No Hit
GTGCTGTCTCTCTTTGATGGATTTAATTTCACGGACATAATTACTGGGGA70.1330039901197036No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA70.1330039901197036No Hit
GTGCTGTCTCTCTTTGATGGACAGGTGCTGTCTCTCTTTGATGGACAGGT70.1330039901197036No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCTTCTCTTTTGGTTGTTACGCAGAC70.1330039901197036No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA60.11400342010260307No Hit
GCAAAAGTTCTCAGGGATGTCCCAACCCTGTGATGATTGATGCTTACGCA60.11400342010260307No Hit
GTGCTGTCTCTCTTTGATGGAGTGCTGTCTCTCTTTGATGGGGTGCTGTC60.11400342010260307No Hit
GTGCTGTCTCTCTTTGATGGACCGACCAGCAACTCTGCGTTGATACCACT60.11400342010260307No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA60.11400342010260307Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG60.11400342010260307No Hit
GCAAAAGTTCTCAGGGATGTCCCCCAAACTGTTATCATTGTCCTCAGTCT60.11400342010260307No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT60.11400342010260307Clontech SMART CDS Primer II A (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAGGAGGAGGAGAAGAAAGGGGTCTGGGAAA60.11400342010260307No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA60.11400342010260307No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGTAGGGCTCCTGGTGCTGA60.11400342010260307No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTTGCGTTGATACCA60.11400342010260307No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC60.11400342010260307No Hit
GTGCTGTCTCTCTTTGATGGAATCTTACCATGTTGGTCAGGCTGGTCTTG60.11400342010260307No Hit
GCAAAAGTTCTCAGGGATGTGTCAAAAGTGAGGTGATTTTTGTGATGATA60.11400342010260307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)