FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5263
Total Bases762.2 kbp
Sequences flagged as poor quality0
Sequence length138-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA152528.97586927607828No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA53010.070302109063272No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3506.65019950598518Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT2665.054151624548736No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC971.8430552916587497No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC871.6530495914877448No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA851.6150484514535435No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT801.520045601368041No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA601.1400342010260307No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC571.083032490974729Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA561.0640319209576288Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC561.0640319209576288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA470.8930267908037242No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA440.8360250807524225Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA400.7600228006840205No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC360.6840205206156185No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCTCTATG330.6270188105643169No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT330.6270188105643169No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA300.5700171005130154No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA280.5320159604788144No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCTCTAT280.5320159604788144No Hit
CAACCAAAAGAGAAGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC260.49401482044461337Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC260.49401482044461337No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT260.49401482044461337No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA240.4560136804104123No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT230.4370131103933118No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT230.4370131103933118No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC230.4370131103933118No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC190.36101083032490977Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA190.36101083032490977No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA180.34201026030780923No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA170.3230096902907087No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCTCTA170.3230096902907087No Hit
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA140.2660079802394072No Hit
CAACCAAAAGAGAAGGAGGCACATCCATAGCCGTCTCCTGTCAAATCAAG130.24700741022230668No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGCTGC120.22800684020520615No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA120.22800684020520615No Hit
CAACCAAAAGAGAAGGAGGCACTAGGGATCGTCCGCAGGATTGGGACTGA110.20900627018810564No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA110.20900627018810564No Hit
GCATCAATCATCACAGGGTTGGTCTTTCTGGCACAGCTAGCCCCCATTCC110.20900627018810564No Hit
CAACCAAAAAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG100.19000570017100513No Hit
CAACCAAAAGAGAAGGAGGCACGGAAGAGTCAGTACAAGGCCCAAGATAG100.19000570017100513No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG90.17100513015390462No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATCAAAG90.17100513015390462No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG90.17100513015390462No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGTGGT90.17100513015390462Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACAGAGAGGGAAACAACTCCACCAAGGTTC90.17100513015390462No Hit
CAACCAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTAG90.17100513015390462No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA80.1520045601368041No Hit
CAACCAAAAGAGAAGGAGGCTGGTGGGAAGGCACATTAAATGGGAGAACA80.1520045601368041No Hit
CAACCAAAAGAGAAGGAGGCACCCTTGCTTGAAATGCGTGTCGACACGTG80.1520045601368041No Hit
CAACCAAAAGAGAAGGAGGCACGGACATCCCTGAGAACTTTTGCCCTCTA80.1520045601368041No Hit
GCATCAATCATCACAGGGTTGGTAAGGGAGGGCCCAGACCGAAGAAGGTT70.1330039901197036No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA70.1330039901197036No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATAA70.1330039901197036No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA70.1330039901197036No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA60.11400342010260307No Hit
CATCAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTAGA60.11400342010260307No Hit
CAACCAAAAGAGAAGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA60.11400342010260307No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCCATCAAA60.11400342010260307No Hit
GCATCAATCATCACAGGGTTGGGACATCCCTGAGAACTTTTGCCCTCTAT60.11400342010260307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)