FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17887
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA681538.10029630457875No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA312317.459607536199474No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC7063.947000614971767No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC4922.7506009951361325No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG4112.2977581483759155No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG3932.1971264046514225No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC3001.6771957287415442No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA2651.4815228937216973No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT2351.3138033208475428Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA1780.9951361323866494No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA1450.8106446022250796No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG1450.8106446022250796No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1310.732375468217141No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG960.536702633197294No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC800.4472521943310785No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA760.4248895846145245No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG750.41929893218538605No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG720.4025269748979706No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT710.3969363224688321No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA710.3969363224688321No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA670.3745737127522782No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT650.36339240789400123Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC640.35780175546486276No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA580.3242578408900319No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC580.3242578408900319Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT560.3130765360317549No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG510.2851232738860625No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC430.24039805445295465No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA400.22362609716553924No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG390.2180354447364007No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCCTCC330.18449153016156986No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTTACGCAG320.17890087773243138No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA320.17890087773243138No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA310.17331022530329288No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA300.16771957287415443No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG290.16212892044501595No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG280.15653826801587745No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA260.1453569631576005No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.1341756582993235No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG230.12858500587018507No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA220.12299435344104657No Hit
GTGCTGTCTCTCTTTGATGGAATCTGCGTTGATACCACTGCTTCCCATGT200.11181304858276962Clontech Universal Primer Mix Long (95% over 23bp)
GGTGTTTGGGATGGAATGGTATCAACGCAGGTGGTTCGCAGAAGCAGCAG200.11181304858276962No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAT200.11181304858276962No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG200.11181304858276962No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC190.10622239615363112No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC180.10063174372449266No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)