FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8645
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT222825.772122614227875No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA177720.55523423944477No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG2102.42914979757085No Hit
GCAAAAGTTCTCAGGGATGTGAGCGTTCGAGCAACTCTGCGTTGATACCA2102.42914979757085No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG1992.3019086176980914No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1942.2440717177559284No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA1932.232504337767496No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1752.0242914979757085No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA1401.6194331983805668No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1191.376518218623482Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA1031.1914401388085598No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT720.8328513591671487No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT610.7056101792943898No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA610.7056101792943898No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG600.6940427993059572No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTTACGCAG570.6593406593406593No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA520.6015037593984963No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC480.5552342394447658No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG420.4858299595141701No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC420.4858299595141701No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT420.4858299595141701Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG410.4742625795257374No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA400.46269519953730476No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT350.4048582995951417No Hit
GTGCTGTCTCTCTTTGATGGAATCTGGAAAAATCTAGATTCAAGGCTCCT350.4048582995951417No Hit
GCAAAAGTTCTCAGGGATGTGAGCGTTCGAGCAACTCTGCGTTGATACCC350.4048582995951417No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA330.38172353961827643No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA290.335454019664546No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA280.32388663967611336No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC280.32388663967611336Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA220.25448235974551764No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGTTACGC210.24291497975708504No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT210.24291497975708504No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCCT190.21978021978021978No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC190.21978021978021978No Hit
GTGCTGTCTCTCTTTATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT180.20821283979178717No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC180.20821283979178717No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCCTCC150.1735106998264893No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA150.1735106998264893No Hit
GCAAAAGTTCTCAGGGATGTGTCATCCCCAAGCAGCGGTCATCCCCAAGC150.1735106998264893No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA140.16194331983805668No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT140.16194331983805668No Hit
TGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT130.15037593984962408No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA130.15037593984962408No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCCTCCTT130.15037593984962408No Hit
GTGCTGTCTCTCTTTGATGTTACGCAGACCAAGTCTCTGCTACCGTATGG120.13880855986119145No Hit
GTGCTGTCTTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT120.13880855986119145No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAT120.13880855986119145No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGATCCAGGAGTGGGGCCCATT120.13880855986119145No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA120.13880855986119145No Hit
GTGCTGTCTCTCTTTGAGGAATCGCTACAGATCCAGGAGTGGGGCCCATT110.12724117987275882No Hit
GTGCTGTCTCTCTTTGATGGAATCTGCGTTGATACCACTGCTTCCCATGT110.12724117987275882Clontech Universal Primer Mix Long (95% over 23bp)
GTGCTGTCTCTCTTTGATGGAATCAGTAAAGAACCTAACACAGAATGAAT110.12724117987275882No Hit
GTGCTGTCTCTCTTTGTGGAATCGCTACAGATCCAGGAGTGGGGCCCATT110.12724117987275882No Hit
GTGCTGTCTCTCTTTGATGGAATCGTGCCTCCTTCTCTTTTGGTTGTTAC100.11567379988432619No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACGGATCCAGGAGTGGGGCCCAT100.11567379988432619No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC100.11567379988432619No Hit
GTGCTGTCTCTCTTTGATGGACACGGAAATGTTGAATACTCATCTTGTGC100.11567379988432619No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC100.11567379988432619No Hit
GTGCTGTCTCTCTTTGATGGAGGGTGGTCGCAGCAAAGGGTTTGGTTTTG90.10410641989589359No Hit
GTGCTGTCTCCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT90.10410641989589359No Hit
GTGCTGTCTCTCTTTGATGGACGGTGCTGTCTCTCTTTGATGGACGGTGC90.10410641989589359No Hit
GCAAAAGTTCTCAGGGATGTGAGCGTTCGAGCAACCCTGTGATGATTGAT90.10410641989589359No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTTAGTGAGGACC90.10410641989589359No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG90.10410641989589359No Hit
GTGTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT90.10410641989589359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)