FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729
Total Bases250.6 kbp
Sequences flagged as poor quality0
Sequence length135-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA127773.85772122614227No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA693.990746096009254No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG301.735106998264893No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA211.214574898785425No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG191.098901098901099No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG150.8675534991324465No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC110.6362058993637941No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG90.5205320994794679No Hit
GCAAAAGTTCTCAGGGATGTCCAAGCAACTCTGCGTTGATACCACTGCTT90.5205320994794679Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA90.5205320994794679No Hit
GCAAAAGTTCTCAGGGATGTCCAAGCAGCTCTGCGTTGATACCACTGCTT70.4048582995951417Clontech Universal Primer Mix Long (95% over 23bp)
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA70.4048582995951417No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT60.3470213996529786Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA60.3470213996529786No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG60.3470213996529786No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG50.2891844997108155No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAACA50.2891844997108155No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG40.23134759976865238No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG40.23134759976865238No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG40.23134759976865238No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG40.23134759976865238No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG40.23134759976865238No Hit
GGTGTTTGGGATGGAATGGCGATCCACGCAGGTGGTTCGCAGAAGCAGCA40.23134759976865238No Hit
GGTGTTTGGGATGGAATGGTGATCCGCGCAGGTGGTTCGCAGAAGCAGCA40.23134759976865238No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA40.23134759976865238No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGGTGGTTCGCAGAAGCAGCAG40.23134759976865238No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT40.23134759976865238No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA40.23134759976865238No Hit
GGTGTTTGGGATGGAATGGTGATTCACGCAGGTGGTTCGCAGAAGCAGCA30.1735106998264893No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA30.1735106998264893No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA30.1735106998264893No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA30.1735106998264893No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG30.1735106998264893No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA30.1735106998264893No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCGGAAGCAGCA30.1735106998264893No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAACAGCA20.11567379988432619No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGATGGTTCGCAGAAGCAGCA20.11567379988432619No Hit
GGTGTTTGGGATGGAATGGTGTTCCACGCAGGTGGTTCGCAGAAGCAGCA20.11567379988432619No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGATGTACTCTGCGTTGATACCAC20.11567379988432619Clontech SMART CDS Primer II A (95% over 21bp)
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA20.11567379988432619No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG20.11567379988432619No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC20.11567379988432619Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.11567379988432619Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC20.11567379988432619No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.11567379988432619Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA20.11567379988432619No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG20.11567379988432619No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG20.11567379988432619No Hit
GGTGTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAGT20.11567379988432619No Hit
GGTGTTTGGGATGGAATGGTGATCCACGAAGGTGGTTCGCAGAAGCAGCA20.11567379988432619No Hit
GGTGTTTGGGATGGAATGGTGCGGCCACTGATAGGATTACGCAGACCAAG20.11567379988432619No Hit
GGCGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA20.11567379988432619No Hit
GGTGTTTGAGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA20.11567379988432619No Hit
GGTGTTTGGGATGGAATGGTGACCCACGCAGGTGGTTCGCAGAAGCAGCA20.11567379988432619No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)