FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349
Total Bases50.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA11432.664756446991404No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA4412.607449856733524No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA205.730659025787966No Hit
GCAAAAGTTCTCAGGGATGTAAGCAACTCTGCGTTGATACCACTGCTTCC185.157593123209169Clontech Universal Primer Mix Long (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC164.584527220630372No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA113.151862464183381No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT41.146131805157593No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG30.8595988538681949No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC30.8595988538681949No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG30.8595988538681949No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC30.8595988538681949No Hit
GTGCTGTCTCTCTTTGATGGACACTAGGAGAACCTATTCCTTGAGGCGTG30.8595988538681949No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG30.8595988538681949No Hit
GCAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCACTGCTTCC30.8595988538681949Clontech Universal Primer Mix Long (96% over 25bp)
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC30.8595988538681949No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC30.8595988538681949No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT30.8595988538681949No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG20.5730659025787965No Hit
GCAAAAGTTCTCAGGGATGTAAGCAACTCTGCGTTGATACCCCATGTACT20.5730659025787965No Hit
GTGCTGTCTCTCTTTGATGGAGCAACTCTGCGTTGATACCACTGCTTCCC20.5730659025787965Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA20.5730659025787965No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA20.5730659025787965No Hit
GCAAAAGTTCTCAGGGATGTAAGCAACTCTGCGTTGATACCACCCCATGT20.5730659025787965No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG20.5730659025787965No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA20.5730659025787965No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAC20.5730659025787965No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGAGACCCCC10.28653295128939826No Hit
CTAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGG10.28653295128939826No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC10.28653295128939826No Hit
GTGCTGTCTTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA10.28653295128939826No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAACA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCCTCC10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGAATCTGCGTTGATACCACTGCTTCCCATGT10.28653295128939826Clontech Universal Primer Mix Long (95% over 23bp)
CAAAAGTTCTCAGGGATGTAAGCAACTCTGCGTTGGTACCACTGCTTCCC10.28653295128939826Clontech Universal Primer Mix Long (95% over 23bp)
CTCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAAG10.28653295128939826No Hit
GCAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCTCCCATGTA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTTGTGCTGAAGGA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGTTCCTGGTGCTGAAGGA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA10.28653295128939826No Hit
GGTGTTTGGGATGGAATGGTGATCTACGCAGGTGGTTCGCAGAAGCAGCA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGTGGTCATCCCCAAGCAGCTGCGTTGATACC10.28653295128939826No Hit
GTGCTGTCTCTTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGAC10.28653295128939826No Hit
GCAAAAGTTCTCAGGGATGTAAGCAACTCTGAGTTGATACCACCCCATGT10.28653295128939826No Hit
GGTGTTGGGATGGAATGGCGATCCACGCAGGTGGTTCGCAGAAGCAGCAG10.28653295128939826No Hit
GCAAAAGTTCTCAGGGATGTAAGCAACTCAGCGTTGATACCCCATCTACT10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGACTTCTGAATTCCTTTTCCATATTATCTTG10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGTTACGC10.28653295128939826No Hit
GCAAAAGTTCTCAGGGATGTAAGCAACTCTGCGTTGATATCCCATGTACT10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGACAGCCCCCCCATGTACTCTGCGTTGATAC10.28653295128939826No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTTGCAGAAGCAGCA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGGCTCTCTTTGATGGGGGTCGTG10.28653295128939826No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGAATCTATGTACCAAACCAAGTCAGTAAGGT10.28653295128939826No Hit
GCAAAAGTTCTCAGGGATGTGTGCGGTCATCCCCAAGCAGCTTAGGGAAC10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGACAACTGCTGGGATAGCAATGAAATACAGA10.28653295128939826No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATAACACTGCTTCCCATGT10.28653295128939826Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGGGTGCTGTCTCTCTTTGATGTAGGGTGA10.28653295128939826No Hit
GTGCTGTTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTACTCTTTTGGTTGTTACGCAGA10.28653295128939826No Hit
GCAAAAGTTCTCAGGGATGTCCCCCCATGTACTCTGCGTTGATACCACTG10.28653295128939826Clontech SMART CDS Primer II A (95% over 23bp)
GTGCTGTCTCTCTTTGATGGAGTGCGGTCCTCCCCATGTACTCTGCGTTG10.28653295128939826No Hit
CAAAAGTTCTCAGGGATGTAAGCAACTCTGCGTTGATACCATCCCATGTA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGAATCACTACAGGGCTCCTGGTGCTGAAGGA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGTCCTCACTTTGCTGAACTTTTGCCTCCCAT10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGAGCAACTCTGCGTTGATACCCCATGTACTC10.28653295128939826No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGCGCAGGGCTACTGGTGCTGA10.28653295128939826No Hit
CAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCCCATGTACTC10.28653295128939826No Hit
GCAAAAGTTCTCAGGGATGTAAGCAACTCTGCGTGATACCACTGCTTCCC10.28653295128939826No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGGTCGCAGAAGCAGCA10.28653295128939826No Hit
GGTGCTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA10.28653295128939826No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGGAGGA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGATACCCCCAT10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGATCCCACTTTCACAAGCTTCCCATGTACTC10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTTTGTTACGCAGACC10.28653295128939826No Hit
GCAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCACTGCCTTC10.28653295128939826Clontech Universal Primer Mix Long (95% over 23bp)
GTGCTGTCTCTTTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGAGCAACTCTGCGTTGATACCACTGCTCCCA10.28653295128939826Clontech SMART CDS Primer II A (95% over 23bp)
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTATGGATGTTACGCAGA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGATGCCTCCTTCCTTTTGGTTGTTACGCAGA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTCTCTCTC10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCCCAT10.28653295128939826No Hit
CTCCAAGTAACGGTGCTGTCCCTCTTTGCGGCCACTGATAGGATTACGCA10.28653295128939826No Hit
GCAAAAGTTCTCGGGATGTAAGCAACTCTGCGTTGATACCACTGCTTCCC10.28653295128939826Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGAGTGCGGTCATCCCCATGTACTCTGCGTTG10.28653295128939826No Hit
GGTGTTTGAGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA10.28653295128939826No Hit
GGTGTTTGGGATGGAATGGTGATACACGCAGGTGGTTCGCAGAAGCAGCA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCGCTTTGATGGGTGATGTCTG10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTTAATGACCCCC10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA10.28653295128939826No Hit
GCAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCACTGCCCAT10.28653295128939826Clontech SMART CDS Primer II A (95% over 22bp)
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA10.28653295128939826No Hit
GTGCCGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA10.28653295128939826No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA10.28653295128939826No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCGTTGATGGTGCTGTCTCTC10.28653295128939826No Hit
GTGCTGTCTCTCTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACTG10.28653295128939826No Hit
GGTGTTTGGGATGGAATGGTGATCCACTCAGGTGGTTCGCAGAAGCAGCA10.28653295128939826No Hit

[FAIL]Adapter Content

Adapter graph