FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8573
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA193222.535868424122242No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA120714.079085500991484No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT102711.97947043042109No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT5065.902251253936779No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4315.027411641199113Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA4054.72413390878339No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA3704.315875422839146No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA861.0031494226058555Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC730.8515105563979938Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA690.8048524437186516Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC670.7815233873789805Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGGGTTGGGGGCAAGGTCATAGATCAACGACATCCC630.7348652746996384No Hit
CAACCAAAAGAGAAGGAGGCACGGACATCCCTGAGAACTTTTGCCCTCTA570.6648781056806252No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC490.571561880321941No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG440.5132392394727633No Hit
CAACCAAAAGAGAAGGAGGACATCCCTGAGAACTTTTGCCCTCTATGTAG430.5015747113029277No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG410.47824565496325677No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT340.396593957774408No Hit
CAACCAAAAGAGAAGGAGGCACAATGCAAAGGGGGTCTCTTCGGATTTTG280.32660678875539484No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC270.3149422605855593No Hit
CAACCAAAAGAGAAGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG260.3032777324157238No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC220.25661961973638164No Hit
CAACCAAAAGGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG200.2332905633967106No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG200.2332905633967106No Hit
CAACCAAAAGAGAAGGAGGCCATCCCTGAGAACTTTTGCCCTCTATGTAG200.2332905633967106No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC190.2216260352268751Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCTGGAGAGCCAGGACATCCCTGAGAACTTTT190.2216260352268751No Hit
CAACCAAAAGAGAAGGAGCACCACAGGTTTCTGGATCCAGCCCCTGACAG160.1866324507173685No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC150.17496792254753293No Hit
CAACCAAAAGAGAAGGAGGCACTTGTTCATCGTATATAGTGGTGCAAGCT140.16330339437769742No Hit
CAACCAAAAGGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG140.16330339437769742No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA130.1516388662078619No Hit
CAACCAAAAGAGAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTAT130.1516388662078619No Hit
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA130.1516388662078619No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG130.1516388662078619No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG130.1516388662078619No Hit
CAACCAAAAGAGAAGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG130.1516388662078619No Hit
CAACCAAAAGAGAAGGAGGAGGAGGAAGAGGAGGAGAAGGTAGCTACAGC130.1516388662078619No Hit
CAACCAAAAGAGAAGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC120.13997433803802636Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTCTGGATCCAGCCCCTGACA120.13997433803802636No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA120.13997433803802636No Hit
CACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG120.13997433803802636No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG110.12830980986819082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA110.12830980986819082No Hit
CAACCAAAAAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG100.1166452816983553No Hit
CAACCAAAAGAGAAGGAGGCACAGGTGGCGGGGGCGGGTTGGGCGGGTAG100.1166452816983553No Hit
CAACCAAAAGAAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG100.1166452816983553No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGGA100.1166452816983553Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAACCAAAAGAGAAGGAGGCACCAGCAGAATCCTGGTTACACAGACTGAC90.10498075352851977No Hit
CAACCAAAAGAGAAGGAGGCACAGACAAGTGGAGAAAATGAAATGTTAAA90.10498075352851977No Hit
GCATCAATCATCACAGGGTTGGCAGACAGCTTTGCTCTGGAAGGTTGTTG90.10498075352851977No Hit
CAACCAAAAGAGAAGGAGGGACATCCCTGAGAACTTTTGCCCTCTATGTA90.10498075352851977No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA90.10498075352851977No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA90.10498075352851977No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCTCTAT90.10498075352851977No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG90.10498075352851977No Hit
CAACCAAAAGAGAAGGGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG90.10498075352851977No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG90.10498075352851977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)