FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414
Total Bases204.9 kbp
Sequences flagged as poor quality0
Sequence length135-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT66947.31258840169731No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA30621.64073550212164No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA402.828854314002829No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA302.1216407355021216Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT221.5558698727015559No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC211.4851485148514851No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC181.272984441301273No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC130.9193776520509194Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC110.7779349363507779No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA90.6364922206506365No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA80.5657708628005658No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC70.49504950495049505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA60.4243281471004243No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA60.4243281471004243Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC60.4243281471004243No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT60.4243281471004243No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA60.4243281471004243No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA50.3536067892503536No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT50.3536067892503536No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA50.3536067892503536No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT40.2828854314002829No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.21216407355021216No Hit
TCCTATCAGTGACCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT30.21216407355021216No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA30.21216407355021216No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT30.21216407355021216No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC30.21216407355021216Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG30.21216407355021216No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT30.21216407355021216No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA30.21216407355021216No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.21216407355021216No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT30.21216407355021216No Hit
CAACCAAAAGAGAAGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG20.14144271570014144No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.14144271570014144Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAGCAAAATGAAAGGAGGCA20.14144271570014144No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT20.14144271570014144No Hit
CATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC20.14144271570014144Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.14144271570014144No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA20.14144271570014144No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAGCA20.14144271570014144Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCAGCACCGTACTTGGAGCCTCTATGTAGAACCATGTCG20.14144271570014144No Hit
TCCTATCAGTGGCCTCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.14144271570014144No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT20.14144271570014144No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCTCTGGGCTTCACTTACTCT20.14144271570014144No Hit
TCCTATCAGTGGCCACAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.14144271570014144No Hit
TCCTATCAGTGGCGGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.14144271570014144No Hit
TCATATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.14144271570014144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)