FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28706
Total Bases4.1 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1236143.06068417752386No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG384213.383961541141225No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA16825.85940221556469No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC8182.8495784853340766No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT6022.097122552776423Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGACCGACCAGCAACTCTGCGTTGATACCACC5681.9786804152442No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC4041.4073712812652408No Hit
GTGCTGTCTCTCTTTGATGGACCGACCAGCAACTCTGCGTTGATACCACT3871.3481502124991291No Hit
GTGCTGTCTCTCTTTGATGGACCGACCAGCAACTCTGCGTTGATACCCCA2670.9301191388559883No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG2590.9022504006131122No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG2480.8639308855291578No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG2390.832578555005922No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA1600.557374764857521No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT1320.4598341810074549Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1230.42848185048421933No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1150.4006131122413433No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1000.34835922803595065No Hit
GTGCTGTCTCTCTTTGATGGACCGACCAGCAACTCTGCGTTGATACCATC940.32745767435379364No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA740.2577858287466035No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG730.254302236466244No Hit
GCAAAAGTTCTCAGGGATGTCGGCAGAGGCCCAACAATGGGCTGGCCTCT700.24385145962516544No Hit
GTGCTGTCTCTCTTTGATGGACCGACCAGCAACCCTGTGATGATTGATGC700.24385145962516544No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC670.23340068278408696Clontech SMART CDS Primer II A (95% over 24bp)
GCAAAAGTTCTCAGGGATGTCCCATAAATGCTTTCAATAATTGTTTTGGT650.22643349822336792No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCAGCAACTCTGCGTTGATACCACT580.20204835226085138No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGGTGGTTCGCAGAAGCAGCAG540.18811398313941335No Hit
GCAAAAGTTCTCAGGGATGTCCCAGCGCTGCTCCCACTGCACCAGGCTCG470.16372883717689682No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGATGGACCGACCAGCAACTCTGC460.1602452448965373No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT440.15327806033581828No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA430.1497944680554588No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG430.1497944680554588No Hit
GCAAAAGTTCTCAGGGATGTCCGTCTCTACACAGTACTGAAATGGAAAAA420.1463108757750993No Hit
GTGCTGTCTCTCTTTGATGGACCGACCAACCCTGTGATGATTGATGCTTA420.1463108757750993No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG400.13934369121438026No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA400.13934369121438026No Hit
GTGCTGTCTCTCTTTGATGGACCGACCAGCAACTCTGCGTTGATACCCTG390.13586009893402076No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA390.13586009893402076No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG390.13586009893402076No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATCTGGGACTCTGCGTTGATACCAC390.13586009893402076No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT380.13237650665366127No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT380.13237650665366127No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA370.12889291437330175No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA360.12540932209294225Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA360.12540932209294225No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG350.12192572981258272No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC350.12192572981258272No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA340.11844213753222323No Hit
GCAAAAGTTCTCAGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA340.11844213753222323No Hit
GTGCTGTCTCTCTTTGATGGACCGACCAGCAACTCTGCGTTGATACCAGC340.11844213753222323No Hit
GCAAAAGTTCTCAGGGATGTCCTCCTTCTCTTTTGGTTGTTACGCAGACC320.11147495297150421No Hit
GCAAAAGTTCTCAGGGATGTGGATGGAAGCATGGTGCCTCCTTCTCTTTT320.11147495297150421No Hit
GCAAAAGTTCTCAGGGATGTCCGGGTGCCAAAGGTAAACAGGGGGAGGAA310.10799136069114472No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT300.10450776841078521No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA300.10450776841078521No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA290.10102417613042569No Hit
GTGCTGTCTCTCTTTGATGGACCGACCAGCCCATGTACTCTGCGTTGATA290.10102417613042569No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)