FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2084
Total Bases302.1 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA128061.42034548944337No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG663.166986564299424No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA653.1190019193857963No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG643.071017274472169No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT331.583493282149712No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA231.1036468330134357No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA221.055662188099808No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA221.055662188099808No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC180.8637236084452975No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA170.8157389635316697No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC160.7677543186180422No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG160.7677543186180422No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA150.7197696737044146No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG140.6717850287907869No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA130.6238003838771593No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA110.527831094049904No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA110.527831094049904No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA100.47984644913627633No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCGGGGCTCCTGGTGCTGA80.3838771593090211No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT80.3838771593090211No Hit
CTCCAAGTAACGGTGCTGCCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA80.3838771593090211No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA80.3838771593090211No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG80.3838771593090211No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGTTGGTGCAGGGCTCCTGGTGCTGA80.3838771593090211No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT70.33589251439539347No Hit
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA60.28790786948176583No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA60.28790786948176583No Hit
CTCCAAGTAACGGTGCTGTCTGTTGGCTGGTGCAGGGCTCCTGGTGCTGA60.28790786948176583No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT50.23992322456813817Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA50.23992322456813817No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA50.23992322456813817No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGTAGGGCTCCTGGTGCTGA40.19193857965451055No Hit
CTCCAAGTAACGGTGCGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA40.19193857965451055No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTA40.19193857965451055No Hit
CTCCAAGTAACGGTGCTGTCTACTGGCTGGTGCAGGGCTCCTGGTGCTGA40.19193857965451055No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGAGTACATGGGAAGCAGTGGT30.14395393474088292Clontech SMARTer II A Oligonucleotide (95% over 21bp)
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCCCCTGGTGCTGA30.14395393474088292No Hit
CTCCAAGTAACGGTGCGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG30.14395393474088292No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAAGTGGTTCGCAGAAGCAGCA30.14395393474088292No Hit
CTCCAAGTAACGGTGTTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA30.14395393474088292No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC30.14395393474088292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)