FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987
Total Bases288 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA158579.768495218923No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG190.9562154001006542No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC150.754906894816306No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG150.754906894816306No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG140.7045797684952189No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG120.6039255158530448No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT110.5535983895319577Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA110.5535983895319577No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG100.5032712632108707No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG90.45294413688978363No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG70.35228988424760943No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG70.35228988424760943No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG70.35228988424760943No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAACAGCA60.3019627579265224No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA60.3019627579265224No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGGTGGTTCGCAGAAGCAGCAG60.3019627579265224No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA60.3019627579265224No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG50.25163563160543534No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA50.25163563160543534No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA50.25163563160543534No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG50.25163563160543534No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAACA40.20130850528434827No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC40.20130850528434827No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA40.20130850528434827No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG40.20130850528434827No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCGGAAGCAGCA40.20130850528434827No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC40.20130850528434827No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA40.20130850528434827No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTCCGCAGAAGCAGCA40.20130850528434827No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGCTCGCAGAAGCAGCA30.1509813789632612No Hit
GGTGTTTGGGATGGAATGGTAATCCACGCAGGTGGTTCGCAGAAGCAGCA30.1509813789632612No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAAAAGCAGCA30.1509813789632612No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG30.1509813789632612No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA30.1509813789632612No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAGGCAGCA30.1509813789632612No Hit
GGTGTTTGGGATGGAATGGTGACCCACGCAGGTGGTTCGCAGAAGCAGCA30.1509813789632612No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG20.10065425264217413No Hit
GGTGTTTGGGATGGAATGGTGATTCACGCAGGTGGTTCGCAGAAGCAGCA20.10065425264217413No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCATGT20.10065425264217413No Hit
GGTGTTTGGGATGGAATGGTGTTCCACGCAGGTGGTTCGCAGAAGCAGCA20.10065425264217413No Hit
GCAAAAGTTCTCAGGGATGGCCTCCTTCTCTTTTGGTTGTTACGCAGACC20.10065425264217413No Hit
GGTGTTTGGGATGGAATGGTGATCAACGCAGGTGGTTCGCAGAAGCAGCA20.10065425264217413No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC20.10065425264217413No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT20.10065425264217413No Hit
GGTGTTTGGGATGGAATGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG20.10065425264217413No Hit
GTGCTGTCTCTCTTTGATGGACAGCCCCCCCATGTACTCTGCGTTGATAC20.10065425264217413No Hit
GGTGTTTGGGATGGAATGGCGATCCACGCAGGTGGTTCGCAGAAGCAGCA20.10065425264217413No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTTGCAGAAGCAGCA20.10065425264217413No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.10065425264217413Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTTGGGATGGAATGGTGATCCCCGCAGGTGGTTCGCAGAAGCAGCA20.10065425264217413No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGTAGCAGCA20.10065425264217413No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGATTCGCAGAAGCAGCA20.10065425264217413No Hit
CTCCAAGTAACGGTGCTGCCTCACTTTGCTGAACTCTTAGCAACTCTGCG20.10065425264217413No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCACGTGGTTCGCAGAAGCAGCA20.10065425264217413No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTAGTTCGCAGAAGCAGCA20.10065425264217413No Hit
GGTGTTTGGGATGGAATGGTGATCCGCGCAGGTGGTTCGCAGAAGCAGCA20.10065425264217413No Hit
GGTGTTTGGGACGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA20.10065425264217413No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT20.10065425264217413No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT20.10065425264217413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)