FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5001
Total Bases721.5 kbp
Sequences flagged as poor quality0
Sequence length139-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT110422.075584883023396No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT102120.415916816636674No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA50310.057988402319536No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA2745.478904219156169No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT1873.739252149570086No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA1773.5392921415716856No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA721.4397120575884823Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC721.4397120575884823No Hit
CAACCAAAAGAGAAGGAGGCACGTCTTCCATGGCTGGAGCAGGAGGAAGA460.9198160367926416No Hit
GCATCAATCATCACAGGGTTGGTACACAGGGCCTGCTGGCATTCCTGTGA430.8598280343931213No Hit
CAACCAAAAGAGAAGGAGGCACATCCATAGCCGTCTCCTGTCAAATCAAG260.5198960207958409No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT210.4199160167966407No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC210.4199160167966407Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCGATCGTATCTTCCTCGTGTCCCCCATC190.3799240151969606No Hit
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA180.3599280143971206No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA160.3199360127974405Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACTGAGGGGCCAGAGCAGCCCCCACCACCT150.2999400119976005No Hit
CAACCAAAAGAGAAGGAGGCACCAGGCCTTATCCCCTGACATTTCTAGAC150.2999400119976005No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG130.2599480103979204No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA130.2599480103979204Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGGGTTGGGCACTGCCATCCTGAGACTGCCCCCATC130.2599480103979204No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATTGCGAGATGTCCCTCTT130.2599480103979204No Hit
CAACCAAAAGAGAAGGAGGCTGGGCGTTGCATAGGAAACAAGCTTTGTTT120.23995200959808036No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA110.21995600879824037No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC100.19996000799840033No Hit
GCATCAATCATCACAGGGTTGGTTCGGCTGATCTGGCTGGCTAGGCGGGT100.19996000799840033No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG90.1799640071985603No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC90.1799640071985603Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACAAAGCTGGCAAAGTTGAATAGCTAAACC90.1799640071985603No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC90.1799640071985603No Hit
CAACCAAAAGAGAAGGAGGCTGGAGAGCCAGGACATCCCTGAGAACTTTT90.1799640071985603No Hit
CAACCAAAAGAGAAGGAGGCGAGAAAGGGGGAGAGTAGGAGGGAGAGGGG80.15996800639872025No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG70.13997200559888023No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGGCAGCACCATCAAAGA70.13997200559888023No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA70.13997200559888023No Hit
GCATCAATCATCACAGGGTTGGGCATGGGGACAGTTTTTATAGTACAACT70.13997200559888023No Hit
CAACCAAAAAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG70.13997200559888023No Hit
CAACCAAAAGAGAAGGAGGCAGGGGACATCCCTGAGAACTTTTGCCCTCT70.13997200559888023No Hit
GCATCAATCACACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG70.13997200559888023No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT60.11997600479904018No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG60.11997600479904018No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT60.11997600479904018No Hit
CAACCAAAAGAGAAGGAGGCACTGAGGGGCCAGAGCAGCCCCCCCCACCT60.11997600479904018No Hit
CAACCAAAAGAGAAGGAGGCACAGGTTGCCCATGCAGACCAGCAGCTTCG60.11997600479904018No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA60.11997600479904018No Hit
CATCAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTAGA60.11997600479904018No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGACGTCCCTCTT60.11997600479904018No Hit
CAACCAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTAG60.11997600479904018No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG60.11997600479904018No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)