FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866
Total Bases125.3 kbp
Sequences flagged as poor quality0
Sequence length139-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT60870.20785219399538No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT606.928406466512701No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA303.4642032332563506No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA141.6166281755196306No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT101.1547344110854503No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC91.0392609699769053No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC50.5773672055427251No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA50.5773672055427251No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT40.4618937644341801No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT40.4618937644341801No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA30.3464203233256351Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.3464203233256351No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT30.3464203233256351No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC30.3464203233256351No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.23094688221709006Clontech Universal Primer Mix Long (96% over 26bp)
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.23094688221709006No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTGGAGCCTCTATG20.23094688221709006No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCGTCACTTACTCT20.23094688221709006No Hit
CAACCAAAAGAGAGACAGCACCATCAAAGAGAGACAGCACCATCAAAGAG20.23094688221709006No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT20.23094688221709006No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCC20.23094688221709006No Hit
GCATCAATAATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT20.23094688221709006No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGCCCCTGGGCTTCACTTACTCT20.23094688221709006No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA20.23094688221709006No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGCCCCTGGGCTTAACTTACTCT10.11547344110854503No Hit
GCATCAATCATCACAGGGTTGGACAGAGGGAAAAGTTGAGTTGTGATTCG10.11547344110854503No Hit
CAACAAAAGAGAAGGAAGCACCATCAAAGAGAGACAGCACCATCAAAGAG10.11547344110854503No Hit
GCATCAATCATCACAGGGTTGGTTCGGCTGATCTGGCTGGCTAGGCGGGT10.11547344110854503No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGAGGCAA10.11547344110854503No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCTCTA10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGTCCCTGGGCTTCACTTACTCT10.11547344110854503No Hit
GCATCAATCATCATAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT10.11547344110854503No Hit
CAACCAAAAGAGAAGGAGGAACCATCAAAGAGAGACAGCACCATCAAAGA10.11547344110854503No Hit
TCCTATCAGTTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.11547344110854503No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA10.11547344110854503Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG10.11547344110854503No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGGAGC10.11547344110854503Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCAAATAGTGGAGAGGCCCTAGGCTTCACTTACTCT10.11547344110854503No Hit
TCCTATCAGTGGCCTCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.11547344110854503No Hit
GCATCAATCATCACCGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT10.11547344110854503No Hit
CAACCAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTAG10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGACCTGGGCTTCACTTACTCT10.11547344110854503No Hit
GGGCAAAGGGTAAAGAGAAAGAGGGCAAGTGAACAAAATGAAAGGAGGCA10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT10.11547344110854503No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGGCCTGGGCTTCACTTACTCT10.11547344110854503No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAATCA10.11547344110854503Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGACCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.11547344110854503No Hit
CAACCAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCGCTAAT10.11547344110854503No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTAAGA10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTCACTCT10.11547344110854503No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC10.11547344110854503No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGACCCTCTT10.11547344110854503No Hit
CAACCAAAAGAGAAGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA10.11547344110854503No Hit
GCATCATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.11547344110854503Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGGTTGGGAAAGACAGTATCCATCAAAGAGAGACA10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAAGCCCTGGGCTTCACTTACTCT10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAAAGGCCCCGGGCTTCACTTACTCT10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCTCTGGGCTTCACTTACTCT10.11547344110854503No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA10.11547344110854503No Hit
TCATATCACTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCAATTACTCT10.11547344110854503No Hit
GCATCAATCATCACAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGT10.11547344110854503No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC10.11547344110854503Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.11547344110854503No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG10.11547344110854503No Hit
TCTTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGCTTCACTTACTCTT10.11547344110854503No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCTCTCTT10.11547344110854503No Hit
CCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.11547344110854503No Hit
TCTAGCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCGTCACTTACTCTT10.11547344110854503No Hit
TAATATCAGTGGCAGCAAAGAGGGGAGAGGACCTGGGATTCACTTACTCT10.11547344110854503No Hit
GCATCAATCATCACAGGGTTGGTCTCCATCCTGGGAACTCTAGTAGCTTC10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACGCT10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTTACTTACTCT10.11547344110854503No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.11547344110854503No Hit
CAACCAAAAGAGAAGGAGGCAGTGGAGGAAGTGGGGGTTATGGTGGGAGG10.11547344110854503No Hit
TCCTATCAGTGTCCGCAAAGAGGGGAGAGGCCCTGGGATTCACTTACTCT10.11547344110854503No Hit
GCATATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTGT10.11547344110854503No Hit
CAACCAAAAGAGAAGGAGGCACCGTCAAAGAGAGACAGCACCATCAAAGA10.11547344110854503No Hit
GCATCAATCATCACATCCCTGAGAACTTTTGCCCTCTATGTAGAACCATG10.11547344110854503No Hit
GCATCAATCATCACAGGGTTGGGGACAACAAGGAAGGATTGGGATTCTAT10.11547344110854503No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA10.11547344110854503No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGATATGTCCCTCTT10.11547344110854503No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG10.11547344110854503No Hit
CAACCAAAAGAGAAGGAGGCACAGCTCCTCAGAAGAAGGTCTGAGAAACC10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGATTCACTTACTCT10.11547344110854503No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCATCTT10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCG10.11547344110854503No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC10.11547344110854503No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.11547344110854503No Hit
TCCTATCGGTGGCCGCAAAGTGGGGAGAGGCCCTGGGCTTCACTTACTCT10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCGCTTACTCT10.11547344110854503No Hit
CAACCAAAAGAGAAGGAGGCACAATCAAAGAGAGACAGCACCATCAAAGA10.11547344110854503No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGTAAGCA10.11547344110854503Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGACCTTTTGCCCTCTA10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCGCTGGGCTTCACTTACTCT10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCATTTACTCT10.11547344110854503No Hit
TCCTATCAGTGGCCGCACAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.11547344110854503No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.11547344110854503No Hit
TCCTATCAGAGGCCGCAAAGAGGGGACAGACCCTGGGCTTCACTAACTCT10.11547344110854503No Hit
GCATCAATCATCACAGGGAGACAGCACCATCGAAGAGAGACAGCACCCTC10.11547344110854503No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG10.11547344110854503No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCTTGGGCTTCACTTACTCT10.11547344110854503No Hit
GGGCAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.11547344110854503Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGACTTCACTTACTCT10.11547344110854503No Hit

[WARN]Adapter Content

Adapter graph