FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67142
Total Bases9.7 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC4065160.54481546572935No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA1030415.3465788924965No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA35385.269428971433678No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC4970.7402222156027524No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA4500.670221321974323No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA4150.6180929969318757No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC4090.6091567126388847No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA3850.5734115754669209No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA3120.4646867832355307No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA2860.42596288463256976No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA2460.36638765601262996No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA2100.3127699502546841No Hit
GCAAAAGTTCTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAG2090.31128056953918565No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1980.29489738166870216No Hit
GTGCTGTCTCTCTTTGATGGATCTCAGTGCGGTGATGGTAGCCTTTCTGC1960.29191862023770515No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCACAGAAGC1870.2785141937982187No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1650.24574781805725177No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC1460.21744958446278037No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG1430.21298144231628488No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1400.2085133001697894No Hit
GCAAAAGTTCCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1360.20255577730779545No Hit
GCAAAAGTTCTCAGGGATGTCCTCTTGCTAAGTGGGTGGTTCGCAGAAGC1230.18319382800631498No Hit
GCAAAAGTTCTCAGGGATGTCCACATGGAACTTGTGTGGGTATGGGTCTT1220.18170444729081647No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1130.16830002085133003No Hit
GCAAAAGTTCTCAGGGATGTCGGCAGAGGCCCAACAATGGGCTGGCCTCT1130.16830002085133003No Hit
GCAAAAGTTCTCAGGGATGTCTACATGGAACTTGTGTGGGTATGGGTCTT1040.15489559441184356No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA990.14744869083435108No Hit
GCAAAATTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA910.13553364511036312No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGT830.12361859938637514No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAGGC730.10872479223139019No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGCGGTTCGCAGAAGC710.10574603080039319No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC690.1027672693693962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)