FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754
Total Bases109.2 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT42956.896551724137936No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA557.294429708222812No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA445.835543766578249No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA182.3872679045092835Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT151.989389920424403No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC111.4588859416445623No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT111.4588859416445623No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA91.1936339522546418Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC91.1936339522546418No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC50.6631299734748011Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT40.5305039787798408No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCTCTA30.3978779840848806No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.3978779840848806No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT30.3978779840848806No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT30.3978779840848806No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT30.3978779840848806No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCTCTAT30.3978779840848806No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.2652519893899204No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG20.2652519893899204No Hit
TCCTATCAGTGACCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.2652519893899204No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC20.2652519893899204No Hit
TCCTATCAGTGGACGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.2652519893899204No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.2652519893899204No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT20.2652519893899204No Hit
TCCTATCAGTGGCCGTAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.2652519893899204No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT20.2652519893899204No Hit
TCCTATCAGTGGCCACAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.2652519893899204No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.2652519893899204No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.2652519893899204No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC20.2652519893899204Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC20.2652519893899204Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.2652519893899204No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA20.2652519893899204No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA20.2652519893899204No Hit
TGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCACTCA10.1326259946949602No Hit
GCATCAATCATCACAGGGTACATGGGGCAGTGGTATCAACGCAGAGTACA10.1326259946949602Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TCCTAACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGATTAACTTACTCT10.1326259946949602No Hit
TACTATCAGTGGCCTCAAATAGGGTAGAGGCCCTTGGCATCAATTACTCT10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCACAAAGAGAGACAGCACCCTCTATGTA10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA10.1326259946949602Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT10.1326259946949602No Hit
CAACCAAAAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTAGA10.1326259946949602No Hit
TCCTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTTC10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACAGACCCGGAACTGGAAGGGGAGCTGGAC10.1326259946949602No Hit
CATCAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTAGA10.1326259946949602No Hit
TCCTATCAGTGGCCGCAAAGACGGGAGAGGCCCTGGGCTACACTTACTCT10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCGAGCCTGGTGCAGTGGTATCAACGCAG10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCTCCATC10.1326259946949602No Hit
GGGCAAGGGTGAAGCAGTGGTATCAACGCAGAGTTCAGCAAAGTGAGGCC10.1326259946949602Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTGTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.1326259946949602No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCAGATCTGCATCCACAAACGTGAGCTGA10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCAATAA10.1326259946949602No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCAACATCCCTGAGAACTTTTGCCCTCTA10.1326259946949602No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCTATATC10.1326259946949602No Hit
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCACCATCAAAGAGAGACAGCACCACCAT10.1326259946949602No Hit
CAACCAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCGCTAAT10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTAAGA10.1326259946949602No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTCACTCT10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGTACCATCAAAGA10.1326259946949602No Hit
GCTCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.1326259946949602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCACGTTACTTGGAGCCTCTATGTAGAACCATGTCG10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACTCCATCAAAGAGAGACAGCACCCTCTAT10.1326259946949602No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACAAGCAGTGGTATCAACGCAGAGTACATG10.1326259946949602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.1326259946949602No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGTTTCACTTACTCT10.1326259946949602No Hit
GGGCAAAGGGTGAAGAGAAAGCACCGTTACTTGGAGCCTCTATGTAGAAC10.1326259946949602No Hit
TACTATCAGTGGCCGCAAAGAGGGGATAGGCCCTGGGCTTCACTTACTCT10.1326259946949602No Hit
TCCTATCAGTGGCCACAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT10.1326259946949602No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCACTGGGCTTCACTTACTCT10.1326259946949602No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT10.1326259946949602No Hit
CAACCAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.1326259946949602Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCTCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT10.1326259946949602No Hit
CAACCAAAAGAGAATAGGCATGATTCCATCAAAGAGAGACAGCACCCTCT10.1326259946949602No Hit
CCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.1326259946949602No Hit
GGGCAAAGGGGAAGCAGTGGTATCAACGCAGAGTCCATCAAAGAGAGACA10.1326259946949602Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAGAT10.1326259946949602Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCGTCACTTACTCT10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCAACAAAGAGAGACAGCACCCTCTATGT10.1326259946949602No Hit
GGGCAAAGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAA10.1326259946949602No Hit
TCCTACAGTGGCCGCAAAGAGAGTTACTCCATCTACGTGTACAAGGTGCT10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCCATCAAA10.1326259946949602No Hit
GCATCAATCATCACATCCCTGAGAACTTTTGCCCTCTATGTAGAACCATG10.1326259946949602No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA10.1326259946949602No Hit
TCCTATCAGTGGCCGCAGAGAGGGGAGAGGCCCTGGGCTTCACTAAGGTT10.1326259946949602No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAGATGAACAAAATGAAAGGAGGCA10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCGCCTGCCCAGTGACACATGTTTAACGG10.1326259946949602No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAACGAACAAAATGAAAGGAGGCA10.1326259946949602No Hit
TCCTATCAGTGGCCGCAAAGAGGAGAGAGGCCCTGGGCTTCACTTACTCT10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAGAG10.1326259946949602No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACACAGAGTA10.1326259946949602Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT10.1326259946949602No Hit
GCATCAATCATCACAGGGTACATGGGGAAGCAGTGGTATCAACGCAGAGT10.1326259946949602Clontech Universal Primer Mix Long (100% over 23bp)
TACTATCATTGGCCGCAAAGAGGGGAGAGGCCCTGGTCTTCACTTACTCT10.1326259946949602No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGCT10.1326259946949602Clontech Universal Primer Mix Long (95% over 22bp)
CATCAATCATCACACGGTTGTACTAGAGAAATCGCGAGATGTCCCTCTTT10.1326259946949602No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCCCTTACTCT10.1326259946949602No Hit
TCCTATCGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC10.1326259946949602No Hit
ACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCTCTATGT10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA10.1326259946949602No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCGCTTACTCTT10.1326259946949602No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATCAA10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGGACATCCCTGAG10.1326259946949602Clontech SMARTer II A Oligonucleotide (95% over 22bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCC10.1326259946949602No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC10.1326259946949602No Hit
TCCTATCAGTGGCCGCACCGTTCTTGGAGCCTCTATGTAGAACCATGTTG10.1326259946949602No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA10.1326259946949602No Hit
CAACCAAAAGAGAGACAGCACCATCAAAGAGAGACAGCCCCCTCAAAGAG10.1326259946949602No Hit
CCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTTC10.1326259946949602No Hit
GGGCAAAGGGTGAGAGACAGCACCGTTATTGGAGCCTCTATGTAGAACCA10.1326259946949602No Hit
TAATAACAGTGCCCGCAAAGAGTGTATAGGACCTGGTCTTCACTTACTCT10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAAACAGCACCCTCTATGT10.1326259946949602No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.1326259946949602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAACAGAGACAGCACCATCAAAGA10.1326259946949602No Hit
CAACCAAAAGAAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATG10.1326259946949602No Hit
TCATATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.1326259946949602No Hit
TCCTATCAGTGGCCGCAAAGGGGGGAGAGGCCCTGGGCTTCACTTACTCT10.1326259946949602No Hit
CAACCAAAAGAGAAGGAGGCACTCAAATTCTTAGCGGACATCCCTGAGAA10.1326259946949602No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTAT10.1326259946949602No Hit
CAACCAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGGTCCC10.1326259946949602Clontech SMARTer II A Oligonucleotide (96% over 25bp)

[FAIL]Adapter Content

Adapter graph