FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606
Total Bases232.7 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT58936.674968866749694No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA23414.570361145703611No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1448.966376089663761No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA935.790784557907846Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT744.60772104607721No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA251.5566625155666252No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC231.432129514321295Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC221.36986301369863No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC171.0585305105853051Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA150.933997509339975Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC140.8717310087173101Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC140.8717310087173101No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA120.7471980074719801Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA80.49813200498132004No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA80.49813200498132004No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCTCTAT70.43586550435865506No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA50.311332503113325No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA50.311332503113325No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA40.24906600249066002No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT40.24906600249066002No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGTCCTGGGCTTCACTTACTCT30.18679950186799502No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA30.18679950186799502No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.18679950186799502No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA30.18679950186799502No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA30.18679950186799502No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.18679950186799502No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG30.18679950186799502No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGGAAGGAGGCA20.12453300124533001No Hit
CAACCAAAAGAGAAGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG20.12453300124533001No Hit
CAACCAAAAGAGAAGGAGGCACCACCATCAAAGAGAGACAGCACCACCAT20.12453300124533001No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT20.12453300124533001No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA20.12453300124533001No Hit
GGGCAAAGGGTGAAGAGAAAGCACCGTTACTTGGAGCCTCTATGTAGAAC20.12453300124533001No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCTCTATG20.12453300124533001No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT20.12453300124533001No Hit
CCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.12453300124533001No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.12453300124533001No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGGCAGCACCATCAAAGA20.12453300124533001No Hit
GGGCAAAGGGTGAAGAGAAAGAGGACAAATGAACAAAATGAAAGGAGGCA20.12453300124533001No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAGTGAACAAAATGAAAGGAGGCA20.12453300124533001No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGATTCACTTACTCT20.12453300124533001No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA20.12453300124533001No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTT20.12453300124533001Clontech Universal Primer Mix Long (100% over 23bp)
TCCTATCAGTGGCCGCAAAGGGGGGAGAGGCCCTGGGCTTCACTTACTCT20.12453300124533001No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGCACCATGTC20.12453300124533001No Hit
CAACCAAAAGAAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG20.12453300124533001No Hit
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA20.12453300124533001No Hit
GCATCAATCATCACAGGGTTGGTTGGCACTGCTGATCACACCAAGGGGCT20.12453300124533001No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA20.12453300124533001No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT20.12453300124533001No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCACTGGGCTTCACTTACTCT20.12453300124533001No Hit
CAACCAAAAGAGAAGGATTCCATCAAAGAGAGACAGCACCCTCTATGTAG20.12453300124533001No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.12453300124533001No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA20.12453300124533001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)