FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37820
Total Bases5.4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1562841.32205182443152No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA617416.32469592808038No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC17944.7435219460602855No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA9892.615018508725542No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC9012.382337387625595No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT6971.842940243257536Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT5651.4939185616076152No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC5511.4569011105235325No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG4891.2929666842940244No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG4631.2242199894235855No Hit
GCAAAAGTTCTCAGGGATGTCCTCCTTCTCTTTTGGTTGTTACGCAGACC3160.8355367530407193No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT1870.49444738233738766Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1770.4680063458487572No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1610.42570068746694867No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA1550.4098360655737705No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG1500.39661554732945536No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1310.3463775780010577No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA1220.3225806451612903No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA1190.31464833421470123No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC1160.3067160232681121Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG1130.298783712321523No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT1050.27763088313061873No Hit
GCAAAAGTTCTCAGGGATGGCCTCCTTCTCTTTTGGTTGTTACGCAGACC1000.26441036488630354No Hit
CTCCAAGTAACGGTGCTGTCTGTTGGCTGGTGCAGGGCTCCTGGTGCTGA950.2511898466419884No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA950.2511898466419884No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA880.23268112109994712No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAC850.22474881015335804No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA850.22474881015335804No Hit
GTGCTGTCTCTCTTTGATGGACCAGCAACTCTGCGTTGATACCACTGCTT810.2141723955579059Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA780.20624008461131677No Hit
GCAAAAGTTCTCAGGGATGTCCCTCCTTCTCTTTTGGTTGTTACGCAGAC710.18773135906927552No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG690.18244315177154943No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG620.16393442622950818No Hit
GCAAAAGTTCTCAGGGATGTGCCTCCTTCTCTTTTGGTTGTTACGCAGAC600.15864621893178213No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT600.15864621893178213Clontech SMART CDS Primer II A (96% over 25bp)
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA590.1560021152829191No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG560.14806980433633No Hit
GTGCTGTCTCTCTTTGATGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC500.13220518244315177No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA470.12427287149656266No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA410.10840824960338445No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATCTGGGACTCTGCGTTGATACCAC410.10840824960338445No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA400.10576414595452141No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTTCCGAAAACCAACAAAATAGAAC400.10576414595452141No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC390.10312004230565838No Hit
GCAAAAGTTCTCAGGGATGTCCAAGGAATCTGTGCATTTCAAGAAACAAA380.10047593865679534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)