FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37820
Total Bases5.4 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1552141.039132734003175No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA574715.195663670015866No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA25006.610259122157588No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA23076.099947117927023Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT9742.5753569539925967No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA8872.3453199365415123No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC4721.2480169222633528No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC4581.2109994711792702No Hit
CAACCAAAAGAGAAGGAGGACATCCCTGAGAACTTTTGCCCTCTATGTAG3140.8302485457429931No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3100.819672131147541Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1530.4045478582760444No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT1500.39661554732945536No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1450.38339502908514017No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA1390.36753040719196195No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC1240.32786885245901637Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA1220.3225806451612903No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC1150.3040719196192491No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC1110.29349550502379695No Hit
CAACCAAAAGAGAAGGAGGCCATCCCTGAGAACTTTTGCCCTCTATGTAG1000.26441036488630354No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT990.2617662612374405No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA940.24854574299312532No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT870.2300370174510841No Hit
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA840.222104706504495No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC810.2141723955579059Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA780.20624008461131677No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT780.20624008461131677No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA720.19037546271813854No Hit
CAACCAAAAGAGAAGGAGGGACATCCCTGAGAACTTTTGCCCTCTATGTA690.18244315177154943No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA670.17715494447382338No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT650.1718667371760973No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT640.16922263352723427No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA620.16393442622950818No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG610.16129032258064516No Hit
CAACCAAAAGAGAAGGAGGCACATCCCTGAGAACTTTTGCCCTCTATGTA560.14806980433633No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGGA560.14806980433633Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG550.14542570068746694No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT510.1348492860920148No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA510.1348492860920148Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT420.1110523532522475No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT400.10576414595452141No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA390.10312004230565838No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA390.10312004230565838No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT390.10312004230565838No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)