FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18517
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG508327.450450936976832No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA490426.48377166927688No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA221111.94037911108711No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC6903.7263055570556785No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA5342.8838364745909164No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC4882.635416104120538No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA4632.5004050332127234No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT2401.2961062807150185No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC1030.5562456121401955No Hit
GCAAAAGTTCTCAGGGATGTCCATCACTTTCTGAACTGTCTGTGTGCTCG750.40503321272344334No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC740.3996327698871307No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT740.3996327698871307No Hit
GCAAAAGTTCTCAGGGATGTCCATGGAACACGTGCCTGTCTCTTCCTGGA680.3672301128692553No Hit
GCAAAAGTTCTCAGGGATGTCCCATCACTTTCTGAACTGTCTGTGTGCTC640.34562834152400496No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT570.3078252416698169No Hit
GCAAAAGTTCTCAGGGATGTCCCATAAATGCTTTCAATAATTGTTTTGGT570.3078252416698169No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT540.29162391316087916Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT510.27542258465194147No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA490.2646216989793163No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC460.24842037047037857No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT410.22141815628881567No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT380.20521682777987796No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG320.17281417076200248No Hit
GCAAAAGTTCTCAGGGATGTGCATGGAACACGTGCCTGTCTCTTCCTGGA290.15661284225306477No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT270.14581195658043958No Hit
GCAAAAGTTCTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCT270.14581195658043958No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG250.13501107090781445No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.13501107090781445No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA250.13501107090781445No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT250.13501107090781445No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA240.12961062807150187No Hit
TGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC230.12421018523518929No Hit
GTGCTGTCTCTCTTTATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC220.1188097423988767No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG200.10800885672625155No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC200.10800885672625155No Hit
GCAAAAGTTCTCAGGGATGTCCGTGCCTCCTTCTCTTTTGGTTGTTACGC190.10260841388993898No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)