FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7902
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA251431.81473044798785No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA171021.64009111617312No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA85110.769425461908376No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC6067.668944570994684No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC861.088332067830929No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT740.9364717792963807Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCGGAGATGTACTGGGGGCTGTATAGCCCAC740.9364717792963807No Hit
GCAAAAGTTCTCAGGGATGTCCAACCCTGTGATGATTGATGCTTACGCAG730.9238167552518349No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC710.8985067071627436No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG630.7972665148063782No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA580.7339913945836497No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG490.6200961781827385No Hit
GCAAAAGTTCTCAGGGATGTCCATCACTTTCTGAACTGTCTGTGTGCTCG480.6074411541381929No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG320.40496076942546194No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC320.40496076942546194No Hit
GCAAAAGTTCTCAGGGATGTCCCATCACTTTCTGAACTGTCTGTGTGCTC290.36699569729182485No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT280.3543406732472792No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG260.3290306251581878No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA220.27841052898000507No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA180.22779043280182232No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA170.21513540875727666No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA170.21513540875727666No Hit
GCAAAAGTTCTCAGGGATGTCCGTGCCTCCTTCTCTTTTGGTTGTTACGC160.20248038471273097No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA160.20248038471273097No Hit
TGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC150.18982536066818526No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAA150.18982536066818526No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA150.18982536066818526No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC140.1771703366236396Clontech SMART CDS Primer II A (95% over 24bp)
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG130.1645153125790939No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG130.1645153125790939No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT130.1645153125790939Clontech SMART CDS Primer II A (96% over 25bp)
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC130.1645153125790939No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTCTCTTTTGGTTGTTACGCA110.13920526449000253No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA110.13920526449000253No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT110.13920526449000253Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG110.13920526449000253No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA100.12655024044545685No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTGGTTGTTACGCA100.12655024044545685No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT100.12655024044545685No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC100.12655024044545685No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGTTGTTACGCA90.11389521640091116No Hit
GCAAAAGTTCTCAGGGATGTCCAAGCCACTTTCGTAGTCTATTTTGTGTC90.11389521640091116No Hit
GTGCTGTCCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC90.11389521640091116No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAGGGA90.11389521640091116No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA90.11389521640091116No Hit
GCAAAAGTTCTCAGGGATGTGGTGCCTCCTTCTCTTTTGGTTGTTACGCA80.10124019235636549No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)