FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256
Total Bases37.1 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT16865.625No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA4015.625No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC62.34375No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA41.5625No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.78125No Hit
TCCTATCAGTGGCCGCAATGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.390625No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA10.390625No Hit
TCCTATCAGTTGCCGCAAAGAGGTGAGAGCAACTGGGCTTCACTTAATAT10.390625No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCGTCACATACTAT10.390625No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC10.390625No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.390625No Hit
GGGCAAAGGGGGGAGAGGCCCTGGGCTTCACTTACTCTTCATTCAAGGCA10.390625No Hit
CAACCAAAAGAGGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAGA10.390625No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.390625No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.390625No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCGCTTACTCT10.390625No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTATTCT10.390625No Hit
TCCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.390625No Hit
GGGCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATG10.390625Clontech SMART CDS Primer II A (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCACATCCCTGAGAACTTTTGCCCTCTAT10.390625No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAAGCCCTGGGCTTCACTTACTCT10.390625No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTAGGCTTCACTTACTCT10.390625No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCTCTGGGCTTCACTTACTCT10.390625No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACATACTCT10.390625No Hit
TCCTATCAGTGGCAGCAAATATGGGAGAGTCCATGTTATTCACTTACTCT10.390625No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA10.390625No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA10.390625No Hit
TCCTATCATGGCCACAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.390625No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT10.390625No Hit
TCCTATCAGTGGCCGCAAAGAAGGGAGAGGCCCTGGGCTTCACTTACTCT10.390625No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.390625No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.390625No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA10.390625No Hit
GGAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAAGG10.390625No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.390625No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT10.390625No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT10.390625No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG10.390625No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.390625Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA10.390625No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGTCA10.390625No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)