FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684
Total Bases99.1 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT42862.57309941520468No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA304.385964912280701No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA263.8011695906432745No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA202.923976608187134No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT131.9005847953216373No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC121.7543859649122806No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT111.608187134502924No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC91.3157894736842104No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA40.5847953216374269Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT40.5847953216374269No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.43859649122807015No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTCACTCT30.43859649122807015No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.43859649122807015No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.43859649122807015No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT30.43859649122807015No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA30.43859649122807015No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT20.29239766081871343No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG20.29239766081871343No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA20.29239766081871343No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.29239766081871343No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT20.29239766081871343No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.29239766081871343No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT20.29239766081871343No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT20.29239766081871343No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA20.29239766081871343No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA20.29239766081871343No Hit
TCCTATCAGTGGCAGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.29239766081871343No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG20.29239766081871343No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCTTGGGCTTCACTTACTCT20.29239766081871343No Hit
CAACCAAAGAGAAGGGGCATCATCAAAGAGAGACAGCACCCTCTATGTAG10.14619883040935672No Hit
GCATCAATCATCACAGGGTTGGTTCGGCTGATCTGGCTGGCTAGGCGGGT10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGTGAGAGGCCCTGGGCTTCACTTACTCT10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGTCCCTGGGCTTCACTTACTCT10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGAGGAGAGGCCCTGGGCTTCACTTACTCT10.14619883040935672No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACGTACTCTT10.14619883040935672No Hit
TCCTATCAGTGGCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTCG10.14619883040935672No Hit
CAACCAAAAGAGAAGGAGGAACCATCAAAGAGAGACAGCACCATCAAAGA10.14619883040935672No Hit
GCATCAATCATACAACTGGGGACATCCCTGAGAACTTTTGCCCTCTATGT10.14619883040935672No Hit
CAACCAAAAGAGAAGGAGGCACCCCTCCAACAAAGAGAGAGACCCCCCTC10.14619883040935672No Hit
CACCCAAAAGAGAAGGAGGCACTTTCAGCCGCTTATAGAGGATGGCTCTC10.14619883040935672No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG10.14619883040935672No Hit
CAACCAAAAGAGAAGGAGGCACATGAGCGTTCACCTTGTAAAGAAACGAG10.14619883040935672No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT10.14619883040935672No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTATGGATCCAGACCCTGACA10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGACCTGGGCTTCACTTACTCT10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT10.14619883040935672No Hit
TCCTATCAGTAGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.14619883040935672No Hit
GCATCACTCATCACAGGGTTCGACTCGAGAAATCGCGAGATGTCCCTCTT10.14619883040935672No Hit
TCATAAAATTGCCCGAAAAAAGGGAACAGCCCATGGCATACCATAAAAAT10.14619883040935672No Hit
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA10.14619883040935672No Hit
TCCTATCAGTGACCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.14619883040935672No Hit
GCATCAATCATCACAGGGTTGGACTAGAGAAAACGCGACATGTCCCTATA10.14619883040935672No Hit
TCCTATCAGTGGCCGCAGAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.14619883040935672No Hit
GCATCAATCATCCAGGGTTGGCTGACCATGGACATCCCTGAGAACTTTTG10.14619883040935672No Hit
CAACCAAAAGAGAAGGAGGCACTTTCAGCCGCTTATAGAGGATGGCTCTC10.14619883040935672No Hit
CAACCAAAAGAGAAGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA10.14619883040935672No Hit
TCCTATCGGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGCCTTCACTTACTCT10.14619883040935672No Hit
GCTCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTGT10.14619883040935672No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA10.14619883040935672No Hit
CAACCAAAAGAGAAGGAGGCACCCTCCATCAAAGAGAGACAGCCCCCTCC10.14619883040935672No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC10.14619883040935672Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGAACTGGGCTTCACTTACTCT10.14619883040935672No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA10.14619883040935672No Hit
TCCTATCAGTGGCCCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCACTGGGCTTCACTTACTCT10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGAAGAGGCCCTGGGCTTCACTTACTCT10.14619883040935672No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGCTTCACTTACTCTT10.14619883040935672No Hit
CAACCAAAAGACAATGCGGAACAATCTAAGAGACACAGCAAAATCAAATT10.14619883040935672No Hit
CCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.14619883040935672No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCACTGGGCTTCACTTACTCTT10.14619883040935672No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGTA10.14619883040935672No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGACTCTATGTAGAACCATGTC10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGACAGGCCCTGGGCTACACTTACTCT10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCGTCACTTACTCT10.14619883040935672No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.14619883040935672No Hit
GCATCAATCACACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.14619883040935672Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAA10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACATACTCT10.14619883040935672No Hit
TCATAAATAATCACAGGATAGGACTCTAGAACGCGCGAGATCTCCCTCTG10.14619883040935672No Hit
TACTATCAGTAGACGCAAAGAGGGGAGAGGAACTTCTATTCACTTACTAT10.14619883040935672No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATCCCTGAG10.14619883040935672Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTTT10.14619883040935672No Hit
TGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAAAAAAATGAAAGGAGGCA10.14619883040935672No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGAGGCCCTGGGCTTCACTTACTCTT10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGCGGCCATGGGCTTCACTTACTCT10.14619883040935672No Hit
TCCTATCAGTGGCCGCACCGTTACTTGAGCCTCTATGTAGAACCATGTCG10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT10.14619883040935672No Hit
TCCTATCAGTGCCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.14619883040935672No Hit
GCATCAATCATCACAGGGTTGGGTACTTAGTGAATCCTTCCAATCTTAAA10.14619883040935672No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA10.14619883040935672No Hit
TCTATCAGTGGCCGCAAAGAGGGGACAGGCCCTGGGCTTCACTTACTCTT10.14619883040935672No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCGCTTACTCT10.14619883040935672No Hit
GCATCAATCAACACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT10.14619883040935672No Hit
TCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTTC10.14619883040935672No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAACCCCTGACA10.14619883040935672No Hit
AAACCAAAAAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCTATGTA10.14619883040935672No Hit
GCATCAACATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGATGGGAGAGGCCCTGGGCTTCACTTACTCT10.14619883040935672No Hit
GGGTAAAGGGCGAAGAAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA10.14619883040935672No Hit
CAACCAAAAGAGAAGGAGGCACCAGGGCACTTAAGTGACCAAAGACCTAG10.14619883040935672No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA10.14619883040935672No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.14619883040935672No Hit
TCATATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.14619883040935672No Hit
CAACCAAAAGAGAAGGAGGCACAAGATGAGTATTCAACATTTCCGTGTCC10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGGGGGGAGAGGCCCTGGGCTTCACTTACTCT10.14619883040935672No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCATCACTTACTCT10.14619883040935672No Hit

[WARN]Adapter Content

Adapter graph