FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29857
Total Bases4.3 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC1775359.460093110493354No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA21597.231135077201327No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC12284.112938339417892No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA11093.7143718390997087No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA8192.7430753257192615No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC4981.6679505643567671No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA4861.6277589844927487No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT4181.4000066985966442No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA2230.7468935258063436No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA2180.7301470341963359No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA2170.7267977358743344No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1620.5425863281642496No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA1350.452155273470208No Hit
GCAAAAGTTCTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAG1080.3617242187761664No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA980.32823123555615097No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA910.3047861473021402No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA810.2712931640821248No Hit
GCAAAAGTTCCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA690.2311015842181063No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA670.22440298757410324No Hit
GCAAAATTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA550.18421140771008473No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCACAGAAGC450.1507184244900693No Hit
GCAAAAGTTCTCAGGGATGTCCTCTTGCTAAGTGGGTGGTTCGCAGAAGC420.1406705295240647No Hit
GTAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC390.13062263455806009No Hit
GCAAAAGTTCTCAGGGTGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA390.13062263455806009No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAACA390.13062263455806009No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAA380.12727333623605855No Hit
GCAAAAGTTCTCAGGGATGTCCCATTGCTAAGTGGGTGGTTCGCAGAAGC370.123924037914057No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG350.11722544127005392No Hit
GCAAAAGTTCTCAGGGATGTCCCAGCGCTGCTCCCACTGCACCAGGCTCG330.11052684462605084No Hit
GCAAAAGTTCTCAGGGATGTCTTATTGCTAAGTGGGTGGTTCGCAGAAGC330.11052684462605084No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA330.11052684462605084No Hit
ACAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC320.1071775463040493No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG310.10382824798204776No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)