FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34153
Total Bases4.9 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA2173363.63423418147747No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT16824.924896787983486No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT11483.361344537815126No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA10803.162240505958481No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT6581.9266243082598893No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA5231.5313442450150792No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA4111.2034081925453108No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG3991.16827218692355No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3621.0599361695897873Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG3561.0423681667789069No Hit
CAACCAAAAGAGAAGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG2180.6383041021286564No Hit
CAACCAAAAAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1730.506544081047053No Hit
CAACCAAAAGGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1560.4567680730828917No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1420.41577606652417065No Hit
CAACCAAAAGAAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1310.38356806137088983No Hit
CAACCAAAAGAGAAGGAGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1100.3220800515328083No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA990.28987204637952746Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTCTGGATCCAGCCCCTGACA830.24302403888384622No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG830.24302403888384622No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT760.2225280356044857No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACG630.18446402951424473No Hit
CAACCAAAAGAGAAGGGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG630.18446402951424473No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCCGACA560.1639680262348842No Hit
GCATCAATCATCACAGGGTTGGTTCGGCTGATCTGGCTGGCTAGGCGGGT540.15811202529792406No Hit
AACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG540.15811202529792406No Hit
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA540.15811202529792406No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC540.15811202529792406No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGTCCCTGACA530.155184024829444No Hit
CAACCAAAAGAGAAGGAGGCACCACGGGTTTCTGGATCCAGCCCCTGACA530.155184024829444No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCGGCCCCTGACA440.1288320206131233No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCAGACA430.12590402014464322No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGGCA400.117120018739203No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCCGGATCCAGCCCCTGACA390.11419201827072292No Hit
CAACCAAAAGAGAAGGAGGCACCGCAGGTTTCTGGATCCAGCCCCTGACA370.10833601733376277No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCACTGACA360.1054080168652827No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)