FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584
Total Bases229.6 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA92058.080808080808076No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA1569.848484848484848No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC493.0934343434343434No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA342.146464646464646No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC261.6414141414141417No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA231.452020202020202No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT181.1363636363636365Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG161.0101010101010102No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT140.8838383838383838No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG110.6944444444444444No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG100.6313131313131313No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT80.5050505050505051No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT70.4419191919191919Clontech Universal Primer Mix Long (96% over 26bp)
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG70.4419191919191919No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG50.31565656565656564No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG50.31565656565656564No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC50.31565656565656564Clontech SMART CDS Primer II A (95% over 24bp)
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCGGAAGCAGCA50.31565656565656564No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA50.31565656565656564Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA40.25252525252525254No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA40.25252525252525254No Hit
GGTGTTTGGGATGGAATGGTGATTCACGCAGGTGGTTCGCAGAAGCAGCA30.1893939393939394No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG30.1893939393939394No Hit
GGTGTTTGGGATGGAATGGTAATCCACGCAGGTGGTTCGCAGAAGCAGCA30.1893939393939394No Hit
GGTGTTTGGGATGGAATGGTGACCCACGCAGGTGGTTCGCAGAAGCAGCA30.1893939393939394No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA30.1893939393939394Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC30.1893939393939394No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG30.1893939393939394No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG30.1893939393939394No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGGTGGTTCGCAGAAGCAGCAG30.1893939393939394No Hit
TGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAGT30.1893939393939394No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT20.12626262626262627Clontech SMART CDS Primer II A (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC20.12626262626262627No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA20.12626262626262627No Hit
GGTGTTTGGGATGGAATGGTGATCCGCGCAGGTGGTTCGCAGAAGCAGCA20.12626262626262627No Hit
GTGCTGTCTCTCTTTGAGGGTCATCCCCAAGCAGCTGCGTTGATACCACT20.12626262626262627No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG20.12626262626262627No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA20.12626262626262627No Hit
GTGCTGTCTCTCTTTGATGGAATGCTGTCTCTCTTTGATGGGGTGCTGTC20.12626262626262627No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG20.12626262626262627No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG20.12626262626262627No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAACA20.12626262626262627No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA20.12626262626262627No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGTAGAAGCAGCA20.12626262626262627No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTTTTTGGTTGTTACGCAGAC20.12626262626262627No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGCAGCAGCA20.12626262626262627No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGAAGGGCTCCTGGTGCTGA20.12626262626262627No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT20.12626262626262627No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)