FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5114
Total Bases741.3 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA330364.58740711771608No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT3266.37465780211185No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA2043.989049667579194No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC921.798983183418068No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA911.7794290183809154No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG601.173249902229175No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT490.9581540868204927No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG340.6648416112631991No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG330.6452874462260462No Hit
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCACTGCTTCCC280.5475166210402815Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCACCCCATGTA270.5279624560031287No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.4888541259288229No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG200.3910833007430583No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG160.3128666405944466No Hit
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCACCCCATGTA160.3128666405944466No Hit
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCACTGCTTCCC140.27375831052014077Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCGCAACCCTGTGATGATTGATGCTTACGCA120.23464998044583496No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG120.23464998044583496No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA120.23464998044583496No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG110.21509581540868206No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG110.21509581540868206No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG100.19554165037152915No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG100.19554165037152915No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAACA100.19554165037152915No Hit
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCCCATGTACTC100.19554165037152915No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGGTGGTTCGCAGAAGCAGCAG100.19554165037152915No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA90.17598748533437622No Hit
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCACCCATGTAC90.17598748533437622No Hit
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCCCATGTACTC90.17598748533437622No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAACAGCA80.1564333202972233No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAAGATGTAGCAAC80.1564333202972233No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA80.1564333202972233No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA70.13687915526007038No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG70.13687915526007038No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA70.13687915526007038No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGATCCAGGAGTGGGGCCCATT60.11732499022291748No Hit
GGTGTTTGGGATGGAATGGCGATCCACGCAGGTGGTTCGCAGAAGCAGCA60.11732499022291748No Hit
GGTGTTTGGGATGGAATGGTGATCCGCGCAGGTGGTTCGCAGAAGCAGCA60.11732499022291748No Hit
GGTGTTTGGGATGGAATGTTGATCCACGCAGGTGGTTCGCAGAAGCAGCA60.11732499022291748No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)