FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21286
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT943244.310814619937986No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT506923.813774311754205No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA7483.514046791318237No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA5552.6073475523818472No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA4972.3348679883491497No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA3621.700648313445457No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT2661.2496476557361647No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1990.9348867800432209Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG690.32415672272855395No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC660.3100629521751386No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC630.2959691816217232No Hit
GCATCAATCATCACAGGGTTGGACATCCCTGAGAACTTTTGCCCTCTATG580.2724795640326975No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA550.25838579347928214Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG550.25838579347928214No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG390.18321901719440006No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG370.1738231701587898No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG370.1738231701587898No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA360.1691252466409847Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT350.16442732312317956No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG350.16442732312317956No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG340.15972939960537444No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC340.15972939960537444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACGCCTCAAGGAATAGGTTCTCCTAGTGTC330.1550314760875693No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC330.1550314760875693Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG320.15033355256976416No Hit
GCATCAATCATCACAGGGTTGCACTCGAGAAATCGCGAGATGTCCCTCTT310.14563562905195904No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG290.13623978201634876No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG290.13623978201634876No Hit
GCACAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG280.13154185849854363No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGGGATGTCCCTCTT270.1268439349807385No Hit
GCATCAATCATCACAGGGTTGGTTCGGCTGATCTGGCTGGCTAGGCGGGT270.1268439349807385No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT260.12214601146293337No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGACGTCCCTCTT250.11744808794512825No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.11744808794512825No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC250.11744808794512825No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT240.11275016442732312No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT240.11275016442732312No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG240.11275016442732312No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT230.10805224090951801No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.10805224090951801No Hit
GCATCAATCATCACAGGGTTGGACTCGGGAAATCGCGAGATGTCCCTCTT220.10335431739171286No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)