FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486
Total Bases214.7 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT27818.70794078061911No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT18112.18034993270525No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1459.757738896366083No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA1006.7294751009421265No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA885.921938088829071No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA785.248990578734858Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT744.979811574697174No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA291.9515477792732168Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACTAGGGATCGTCCGCAGGATTGGGACTGA271.8169582772543742No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC201.3458950201884252Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC191.278600269179004Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA181.2113055181695829Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACTAGGGATCGTTCTGCCGGATTGGGACTG161.0767160161507403No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC130.8748317631224763No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC120.8075370121130552Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC90.6056527590847914No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG70.47106325706594887No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGACAT50.3364737550471063No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG50.3364737550471063No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC50.3364737550471063No Hit
CAACCAAAAGAGAAGGAGGACATCCCTGAGAACTTTTGCCCTCTATGTAG50.3364737550471063No Hit
CAACCAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTAT40.2691790040376851No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGGA40.2691790040376851Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT40.2691790040376851No Hit
CAACCAAAAGAGAAGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40.2691790040376851Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG30.2018842530282638No Hit
CAACCAAAAGGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTAT30.2018842530282638No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAAACTTTTGCCCTCTAT30.2018842530282638No Hit
CAACCAAAAGAGAAGGAGGCACGTCTCTGAGCCCAGCCTCTGCGGCTCTC30.2018842530282638No Hit
GCATAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30.2018842530282638Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.2018842530282638No Hit
GCATCATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30.2018842530282638Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT30.2018842530282638No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGGAAG30.2018842530282638Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTAT30.2018842530282638No Hit
GCATCATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA30.2018842530282638Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA30.2018842530282638No Hit
GCATCAATCATCACAGGGTTGGCGGACATCCCTGAGAACTTTTGCCCTCT30.2018842530282638No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA30.2018842530282638No Hit
GCATCAATCATCGCAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.13458950201884254Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCGTGGATGAAATCCATCAAAGAGAGACAGCA20.13458950201884254No Hit
CAACCAAAAGAGAAGGAGGCACGATTCCATCAAAGAGAGACAGCACCCTC20.13458950201884254No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGCAGCA20.13458950201884254Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACTCCATCA20.13458950201884254No Hit
GCATCAATCATCACAGGGTACATGGGAGCAGTGGTATCAACGCAGAGTAC20.13458950201884254Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG20.13458950201884254No Hit
CAACCAAAAGAGAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTAT20.13458950201884254No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCGTCACTTACTCT20.13458950201884254No Hit
CAACCAAAAGGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA20.13458950201884254No Hit
CAACCAAAAGAGAAGGAGGCACTATTAATAATCTTGTCACAGCAAAAGAT20.13458950201884254No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA20.13458950201884254No Hit
CAACCAAAAGAGAAGGAGGCACTTTCCATCAAAGAGAGACAGCACCCTCT20.13458950201884254No Hit
CAACCAAAAGAGAAGGAGGGACATCCCTGAGAACTTTTGCCCTCTATGTA20.13458950201884254No Hit
CAACCAAAAGAGAAGAGGCACTAGGGATCGTCCGCAGGATTGGGACTGAT20.13458950201884254No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGTGGT20.13458950201884254Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA20.13458950201884254No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATATCCCTCTT20.13458950201884254No Hit
CAACCAAAAGGAGGAGGCACTAGGGATCGTCCGCAGGATTGGGACTGATA20.13458950201884254No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT20.13458950201884254No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA20.13458950201884254No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG20.13458950201884254No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA20.13458950201884254No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.13458950201884254No Hit
CAACCAAAAGAGAAGGAGGCACAAGATGAGTATTCAACATTTCCGTGTCC20.13458950201884254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)