FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15651
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA675943.18573893041978No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA167610.70858092134688No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC6013.840010222988947No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC5973.814452750622963No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT5313.392754456584244Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA3892.485464187591847No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG2601.6612357037888954No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG2061.3162098268481246No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1781.1373075202862437No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT1631.0414669989138075Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC1530.9775733179988499No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC1260.8050603795284648Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG980.6261580729665835No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA880.562264392051626No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG710.4536451344961983No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG700.4472557664047026No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG570.3641939812152578No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG500.3194684045747876No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA480.30668966839179607No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA410.2619640917513258No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT390.24918535556833432No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG360.23001725129384704No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC330.21084914701935978No Hit
GCAAAAGTTCTCAGGGATGTCCCACTGCTTCCCATGTACTCTGCGTTGAT330.21084914701935978No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAC320.20445977892786402No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT310.19807041083636828No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA310.19807041083636828No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT300.19168104274487255Clontech SMART CDS Primer II A (96% over 25bp)
GCAAAAGTTCTCAGGGATGATACCACTGCTTCCCATGTACTCTGCGTTGA290.1852916746533768No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA290.1852916746533768Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA290.1852916746533768No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG290.1852916746533768No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC280.17890230656188102No Hit
GTGCTGTCTCTCTTTGATGGAATCTGCGTTGATACCACTGCTTCCCATGT250.1597342022873938Clontech Universal Primer Mix Long (95% over 23bp)
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA250.1597342022873938No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTTACGCAG230.14695546610440227No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA230.14695546610440227No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC220.1405660980129065No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA210.13417672992141078No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA210.13417672992141078No Hit
GTGCTGTCTCTCTTTGATGTTACGCAGACCAAGTCTCTGCTACCGTACAT180.11500862564692352No Hit
GTGCTGTCTCTCTTTGATGGACTCTGCGTTGATACCACTGCTTCCCATGT180.11500862564692352Clontech Universal Primer Mix Long (96% over 26bp)
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG180.11500862564692352No Hit
GCAAAAGTTCTCAGGGATGTCCCATGTACTCTGCGTTGATACCACTGCTT170.10861925755542778Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGTCTCTCTTTGATGGACAGCCCCCCCATGTACTCTGCGTTGATAC170.10861925755542778No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATCTGGGACTCTGCGTTGATACCAC170.10861925755542778No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG160.10222988946393201No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)