FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238
Total Bases324.3 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA99944.638069705093834No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA1255.585344057193923No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC944.20017873100983No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA823.6639857015192137No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG421.876675603217158No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC411.8319928507596068No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG311.3851653261840928No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT291.2957998212689903No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT251.1170688114387846Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCGAGCAACTCTGCGTTGATACCACTGCTTC210.938337801608579Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG180.8042895442359249No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA170.7596067917783735No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA150.6702412868632708No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT140.6255585344057194No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT120.5361930294906166Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGTCTACTGGCTGGTGCAGGGCTCCTGGTGCTGA120.5361930294906166No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA120.5361930294906166No Hit
GCAAAAGTTCTCAGGGATTCGAGCAACTCTGCGTTGATACCACTGCTTCC110.49151027703306527Clontech Universal Primer Mix Long (96% over 25bp)
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA110.49151027703306527No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTTACGCAG110.49151027703306527No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG110.49151027703306527No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA110.49151027703306527No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA100.44682752457551383No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG90.40214477211796246No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA80.3574620196604111No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC80.3574620196604111No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC80.3574620196604111Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC80.3574620196604111No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA60.2680965147453083No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA60.2680965147453083No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA50.22341376228775692No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC50.22341376228775692No Hit
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA50.22341376228775692No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAT50.22341376228775692No Hit
CTCCAAGTAACGGGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA50.22341376228775692No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGTTACGC40.17873100983020554No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA40.17873100983020554No Hit
GTGCTGTCTCTCTTTGATGGATGGCCATGTTATCCTCCTCGCCCTTGCTC40.17873100983020554No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGCTTCC40.17873100983020554Clontech Universal Primer Mix Long (96% over 25bp)
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGTAGGGCTCCTGGTGCTGA40.17873100983020554No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA40.17873100983020554No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC40.17873100983020554No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGCGCAGGGCTCCTGGTGCTGA40.17873100983020554No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG40.17873100983020554No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT40.17873100983020554No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC30.13404825737265416No Hit
CTCCAAGACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAAG30.13404825737265416No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA30.13404825737265416No Hit
CTCCAAGTAACGGTGCGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG30.13404825737265416No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCATGTA30.13404825737265416No Hit
GTGCTGTCTCTCTTTGATGGACTCTGCGTTGATACCACTGCTTCCCATGT30.13404825737265416Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGATGTGCCTGTACCACACTGGATGTGCCCGC30.13404825737265416No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC30.13404825737265416No Hit
GCAAAAGTTCTCAGGGATGTCGAGCAACTCTGCGTTGATACCACTGTTCC30.13404825737265416Clontech Universal Primer Mix Long (95% over 22bp)
CTCCAAGTAACGGTGCTGTCTGTTGGCTGGTGCAGGGCTCCTGGTGCTGA30.13404825737265416No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCCATG30.13404825737265416No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA30.13404825737265416No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGATGCTGA30.13404825737265416No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)