FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28070
Total Bases4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1271845.3081581759886No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG592421.10438190238689No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA12544.467402921268258No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC10593.7727110794442464No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC8673.0887068044175274No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA5862.0876380477377983No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT2750.9796936230851443No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG1740.6198788742429641No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG1700.6056287851799074No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1400.4987531172069825No Hit
GTGCTGTCTCTCTTTGATGGAGCGAGGGAAGGTAACAGTTTAGTTCCAAC1190.4239401496259352No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1090.38831492696829356No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG950.3384396152475953No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT870.30993943712148203No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC840.29925187032418954No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT720.25650160313501963No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT680.24225151407196296No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG610.2173138582116138No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT570.2030637691485572Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT540.1923762023512647No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT520.18525115781973636No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG510.18168863555397222No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA500.17812611328820804No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG480.17100106875667972No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG450.16031350195938723No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT380.1353758460990381No Hit
GCAAAAGTTCTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCT370.13181332383327396No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT350.12468827930174563No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG350.12468827930174563No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA350.12468827930174563No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA340.12112575703598148No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT330.11756323477021731No Hit
GCAAAAGTTCTCAGGGATGTCCGTGCCTCCTTCTCTTTTGGTTGTTACGC310.110438190238689No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG300.10687566797292483No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA290.10331314570716067No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)