FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730
Total Bases105.6 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT39754.38356164383562No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA557.534246575342466No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT506.8493150684931505No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA293.9726027397260277Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA233.1506849315068495No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA212.8767123287671232Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC81.095890410958904No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT70.9589041095890412No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC60.821917808219178Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC50.684931506849315Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA40.547945205479452No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC30.410958904109589No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.410958904109589No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC30.410958904109589No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.273972602739726No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA20.273972602739726Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC20.273972602739726Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGTCCTGGGCTTCACTTACTCT20.273972602739726No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT20.273972602739726No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.273972602739726No Hit
TCCAATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.273972602739726No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC20.273972602739726Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG10.136986301369863No Hit
GCATCAATCATCACAGGGTTGGTTCGGCTGATCTGGCTGGCTAGGCGGGT10.136986301369863No Hit
GCATCAATCAGAACAGGGTACGAATCGAGAAAACGCGAGATGTCCAGCTT10.136986301369863No Hit
TCCTATCAGTGTCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.136986301369863No Hit
TCCTATCAGTGGCCGCAAAGAGAGGAGAGGCCCTGGGCTTCACTTACTCT10.136986301369863No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCACCGCAGAGTA10.136986301369863Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCCTATCAGTGGCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTCG10.136986301369863No Hit
TCCTATCAGTGGCAGCAAAGAGGGAAGAGGCCCTGGGCTTCACTTACTCT10.136986301369863No Hit
GGGCAAAGGGTGAAGAGAGAGGGCAAATGAACAAAATGAAAGGAGGCAAG10.136986301369863No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAATAAAATGAAAGGAGGCA10.136986301369863No Hit
GCATCTCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTGT10.136986301369863No Hit
GGGCAAAGGGTGAAGAGAGAGAGGGCAAATGAACAAAATGAAAGGAGGCA10.136986301369863No Hit
GATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.136986301369863Clontech Universal Primer Mix Long (96% over 26bp)
TCCTGTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.136986301369863No Hit
CAACCAAAAGAGAAGTAGTCACCGACATAACTGAGAACTTATGCCCTCTA10.136986301369863No Hit
TCCTATCAGTGGCCCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCTT10.136986301369863No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGATGC10.136986301369863Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.136986301369863Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGCCGCAATGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.136986301369863No Hit
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA10.136986301369863No Hit
GCATCATTCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.136986301369863Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGCCGCAGAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.136986301369863No Hit
CAACAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCAG10.136986301369863Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACGTCCATCAAAGAGAGACAGCACCCTCTA10.136986301369863No Hit
GCTCAACATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTGT10.136986301369863No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCAGGGCTTCACTTACTCT10.136986301369863No Hit
GCATCATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.136986301369863Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCTAAAAATTGTATACCGCAGATTCAGGC10.136986301369863No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCTCTGGGCTTCACTTACTCT10.136986301369863No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA10.136986301369863No Hit
GCATCCAACATCACAGGGTTCGACTCGAGAAATCGAGAGATGTCCCTCTT10.136986301369863No Hit
CAACCAAAAGAGAAGGAGGCACAAGCAGTGGTATCAACGCAGAGTACATG10.136986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.136986301369863No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.136986301369863No Hit
GCATCAATCATCACAGGGATGGACTCGAGAAAACGCGAGATGTCCCACTA10.136986301369863No Hit
TCCTATCAGTGGCCCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.136986301369863No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGACGG10.136986301369863Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGGGCCGCAACGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.136986301369863No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT10.136986301369863No Hit
GGACAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA10.136986301369863No Hit
CAAACAAAGAGAAGGAGTCACCATCAAAGAGATACAGAACCATCAAAGAG10.136986301369863No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTCGGCTTCACTTACTCT10.136986301369863No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACTAAGAGTACATGGGAA10.136986301369863No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCGTGGGCTTCACTTACTCT10.136986301369863No Hit
CAACCAAAAGAGAAGGAGGCTGCTGAGATGGGAAAGGGCTCCTTCAAGTA10.136986301369863No Hit
TCCTATCAGTGGCCACAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.136986301369863No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT10.136986301369863No Hit
GGGCAAATGAAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.136986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGGTTGAACTCGAGAAATCGCGAGATGTCCCTCTT10.136986301369863No Hit
GCAGCAATAATCACAGGGTTCGACACGAGAAATCGAGAGAAGTCCCTCTT10.136986301369863No Hit
TAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT10.136986301369863No Hit
CAACGCAGAGTACGTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.136986301369863Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGAAGCACCGTTACTTGGATCCTCTATGTAGAACCATGTC10.136986301369863No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGGA10.136986301369863Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.136986301369863Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTGAAGAGAAAGAGGACAAATGAACAAAATGAAAGGAGGCA10.136986301369863No Hit
CAACCAAAAGAGAAGGAGGCACTGAGGGTTGCACCCATGTTTTATTATGA10.136986301369863No Hit
CAACCAAAAGAGAAGGAGGCACAGGTAGCTGCAGCAGATGAGAAGAAGTT10.136986301369863No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGTGAGATGTCCCTCTT10.136986301369863No Hit
CAACCAAAGGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAGA10.136986301369863No Hit
GCATCAATCCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT10.136986301369863No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAACGAACAAAATGAAAGGAGGCA10.136986301369863No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGGGGCAA10.136986301369863No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGAAGAGTACATGGGAC10.136986301369863Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGTCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.136986301369863No Hit
GGGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTTCATTCAAGGCC10.136986301369863No Hit
GCATCAATCATAACATGGTTCGACTCGAGAAATCGCGAGATGTAACTCAT10.136986301369863No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATTGCGAGATGTCCCTCTT10.136986301369863No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCTAGATGTCCCTCTT10.136986301369863No Hit
CAACCAAAAGAAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG10.136986301369863No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT10.136986301369863No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAAACTTTTGCCCTCTAT10.136986301369863No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGCT10.136986301369863Clontech Universal Primer Mix Long (95% over 22bp)
CCAACAAACATCACAGCGTAGGACTCGAGAAATCGCGAGATCTCACTCTT10.136986301369863No Hit
GCATCAATCTCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.136986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.136986301369863No Hit
TCCTATCAGTGGCCGCTAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.136986301369863No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGAAGTCCCTCTT10.136986301369863No Hit
GGGCAAAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.136986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.136986301369863No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC10.136986301369863No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGCGCTTCACTTACTCT10.136986301369863No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.136986301369863No Hit
CAACCAAAAGAGAAGGAGGCCATCCCTGAGAACTTTTGCCCTCTATGTAG10.136986301369863No Hit
TCCTAACAGTGGCCGCAAAGAGCGGAGACGCCCAGGGCTTCACATACTCT10.136986301369863No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACACT10.136986301369863No Hit
CAACCAAAAGAGAAGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.136986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAAGTGGGGAGAGGCCCTGGGCTTCACTTACTCT10.136986301369863No Hit
CAACCAAAAGGGGAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG10.136986301369863Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA10.136986301369863No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT10.136986301369863No Hit
GGGCAAAGGGTGACAGCACCGTTACTGGAGCCTCTATGTAGAACCATGTC10.136986301369863No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.136986301369863No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGAAAATGAACAAAATGAAAGGAGGCA10.136986301369863No Hit
TCCTATCAGTGGCAGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT10.136986301369863No Hit
GCATCAATACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.136986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATACAACTGGGCACATCCCTGAGAACTTTTGCCCTCTATGT10.136986301369863No Hit
TCCTATCAGTGGCCGCAAAGAGCCGAGAGGACCAGGACAACACTAAATCA10.136986301369863No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAGTGAAAGGAGGCA10.136986301369863No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGTTATCAACGCAGAGTA10.136986301369863Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[WARN]Adapter Content

Adapter graph