FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33217
Total Bases4.7 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA920627.71472438811452No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT714021.49501761146401No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT543716.368124755396334No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA14794.452539362374687No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA8152.4535629346419No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA7142.149501761146401No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT7082.1314387211367674No Hit
GCATCAATCATCACAGGGTTGGGGGCAAGGTCACAGATCAACAGGATCCC4691.4119276274196948No Hit
CAACCAAAAGAGAAGGAGGCACTAGGGATCGTCCGCAGGATTGGGACTGA2170.6532799470150826No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1960.590059306981365No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC1930.5810277869765482No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1770.5328596802841918No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC920.2769666134810489No Hit
CAACCAAAAGAGAAGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG880.26492458680795977No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC810.24385104013005388No Hit
CAACCAAAAGAGAAGGAGGCACTCAAATTCTTAGCGGACATCCCTGAGAA730.2197669867838757No Hit
CAACCAAAAGGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG610.18364090676460848No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG600.1806304000963362No Hit
CAACCAAAAAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG590.17761989342806395No Hit
CAACCAAAAGAAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG580.17460938675979168No Hit
GCATCAATCATCACAGGGTTGGTTCGGCTGATCTGGCTGGCTAGGCGGGT470.1414938134087967No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT420.12644128006743535No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG400.12042026673089082No Hit
CAACCAAAAGAGAAGGAGGCAGAGGACGAGCTGAGCGCCAGGCACCCGGC390.11740976006261854No Hit
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTCTGGATCCAGCCCCTGACA380.11439925339434627No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG380.11439925339434627No Hit
CAACCAAAAGAGAAGGAGCACCACAGGTTTCTGGATCCAGCCCCTGACAG350.10536773338952946No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT340.10235722672125719No Hit
CAACCAAAAGAGAAGGAGGCGGAGGACGAGCTGAGCGCCAGGCACCCGGC340.10235722672125719No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)