FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39091
Total Bases5.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA1354234.64224501803484No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA923123.614131129927603No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG36509.337187587935842No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC11592.964876825867847No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA7491.9160420557161495No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA6561.678135632242716No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC5301.355810800439999No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT3610.9234862244506408No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT2850.7290680719347165Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC2660.6804635338057353No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG2170.5551149881046789No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG2160.552556854518943No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC2080.5320917858330562No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG1610.4118595073034714No Hit
GTGCTGTCTCTCTTTGATGGAGGCGGCGGTAATGGCGGATGGTGGGTTGT1440.368371236345962No Hit
GCAAAAGTTCTCAGGGATGTCCGTGCCTCCTTCTCTTTTGGTTGTTACGC1260.3223248318027167No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1060.2711621600879998No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA960.24558082423064131No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC930.23790642347343377No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG820.2097669540303395No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC770.1969762861016602Clontech SMART CDS Primer II A (95% over 24bp)
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG760.1944181525159244No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA750.19186001893018853No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA720.184185618172981No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC720.184185618172981No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG670.17139495024430176No Hit
TGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC580.14837174797267913No Hit
GTGCTGTCTCTCTTTATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC530.1355810800439999No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA520.13302294645826404No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT520.13302294645826404Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT490.12534854570105652No Hit
GTGCTGTCTCTCTTTGATGGAATCGGCGGTAATGGCGGATGGTGGGTTGT480.12279041211532066No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG480.12279041211532066No Hit
GTGCTGTCTCTCTTTGAGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC440.11255787777237726No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT420.10744161060090558No Hit
GTGCTGTCTCTCTTTGTGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC420.10744161060090558No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT420.10744161060090558No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA420.10744161060090558No Hit
GCAAAAGTTCTCAGGGATGTCCGCGTTGATACCACTGCTTCCCATGTACT410.10488347701516974Clontech Universal Primer Mix Long (95% over 21bp)
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAGGGA410.10488347701516974No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA400.10232534342943388No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)