FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134
Total Bases164.1 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA29626.102292768959433No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC928.112874779541446No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC494.320987654320987No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG342.9982363315696645No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT332.91005291005291Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG292.5573192239858904No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT252.204585537918871No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT221.9400352733686066Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA221.9400352733686066No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT211.8518518518518516No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA151.3227513227513228No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA121.0582010582010581No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC110.9700176366843033Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG110.9700176366843033No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC90.7936507936507936No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG80.7054673721340388No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC70.6172839506172839No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA70.6172839506172839No Hit
GCAAAAGTTCTCAGGGATGTGTCATCCCCAAGCAGCGGTCATCCCCAAGC70.6172839506172839No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA60.5291005291005291No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC60.5291005291005291No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC60.5291005291005291No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC60.5291005291005291No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTTACGCAG50.4409171075837742No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCATGT50.4409171075837742No Hit
CTCCAAGTAACGGTGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG50.4409171075837742No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG50.4409171075837742No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA50.4409171075837742No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA50.4409171075837742Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA40.3527336860670194No Hit
CTCCAAGTAACGGTGCGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG40.3527336860670194No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA40.3527336860670194Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA40.3527336860670194No Hit
GTGCTGTCTCTCTTTGATGGAGCACCCCCATGTACTCTGCGTTGATACCA30.26455026455026454No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA30.26455026455026454No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGCGCTGA30.26455026455026454No Hit
GCAAAAGTTCTCAGGGATGTCCCCCCATGTACTCTGCGTTGATACCACTG30.26455026455026454Clontech SMART CDS Primer II A (95% over 23bp)
GCAAAAGTTCTCAGGGATGTGTCATCCCCAAGCAGCTGGTCATCCCCAAG30.26455026455026454No Hit
GTGCTGTCTCTCTTTGATGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG30.26455026455026454No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTTAGTGAGGACC30.26455026455026454No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTCCCATGTA30.26455026455026454Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA30.26455026455026454No Hit
GCAAAAGTTCTCAGGGATGTCTGAAAGTACAAGTACACTGTCCTGTAAGA30.26455026455026454No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCCTCCTT30.26455026455026454No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA30.26455026455026454No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA30.26455026455026454No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG30.26455026455026454No Hit
GTGCTGTCTCTCTTTGATGGACTCTGCGTTGATACCACTGCTTCCCATGT30.26455026455026454Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT30.26455026455026454No Hit
GTGCTGTCTCTCTTTGATGGACCGAGGAGCAGAGTCGGTGACTAACCAGT30.26455026455026454No Hit
CTCCAAGTAACGGTGCTAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG20.1763668430335097No Hit
GCAAAAGTTCTCAGGGATGGGTCATCCCCAAGCAGCTTAGTGAGGACCAC20.1763668430335097No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACCCCATGTACTCT20.1763668430335097No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTTAGGGATACCC20.1763668430335097No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGAGTTGATAC20.1763668430335097No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA20.1763668430335097No Hit
GTGCTGTCTCTCTTTGATGGACATCCCCAAGCAGCTTAGTGAGGACCACC20.1763668430335097No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGTGCTGTCTC20.1763668430335097No Hit
GTGCTGTCTCTCTTTGATGGACGGCACCCCATGTACTCTGCGTTGATACC20.1763668430335097No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGGCTC20.1763668430335097No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA20.1763668430335097No Hit
GTGCTGTCTCTCTTTGATGGAATCGTGCCTCCTTCTCTTTTGGTTGTTAC20.1763668430335097No Hit
GCAAAAGTTCTCAGGGATGTGTCATCCCCAAGCAGGGTCATCCCCAAGCA20.1763668430335097No Hit
GTGCTGTCTCTCTTTGATGGATAGTAGGTAGGGACAGTGGGAATCTCGTT20.1763668430335097No Hit
GCAAAAGTTCTCAGGGATGTCCGAAAGTACAAGTACACTGTCCTGTAAGA20.1763668430335097No Hit
GCAAAAGTTCTCAGGGATGTCCGCTGCGTTGATACCACTGCTTCCCATGT20.1763668430335097Clontech Universal Primer Mix Long (95% over 23bp)
GCAAAAGTTCTCAGGGATGTCTGCGTTGATACCACTGCTTCCCATGTACT20.1763668430335097Clontech Universal Primer Mix Long (95% over 24bp)
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA20.1763668430335097No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC20.1763668430335097No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTGGTTGTTACGCAGAC20.1763668430335097No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA20.1763668430335097No Hit
GTGCTGTCTCTCTTTGATGGAGGGTGCTGTCTCTCTTTGATGGAGGGTGC20.1763668430335097No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAG20.1763668430335097No Hit
GCAAAAGTTCTCAGGGATGGGTCATCCCCAAGCAGCTGCGTTGATACCAC20.1763668430335097No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)