FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22055
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1083149.109045567898434No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA231110.478349580593969No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC6633.006121060983904No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC3811.727499433235094No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA3671.6640217637723873No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG3631.6458852867830425No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG3511.591475855815008No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT2891.3103604624801632Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAATCGAAAGGCGGGAAAGGTGGGGAAAAGA2160.9793697574246203No Hit
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCACTGCTTCCC1890.8569485377465427Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1750.793470868283836No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG1470.6665155293584221No Hit
GTGCTGTCTCTCTTTGATGGAATCGAAAAGGGGGAAAGGTGGGGAAAAGA1380.6257084561323962No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA1220.553162548175017No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT1040.4715484017229653Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG990.4488778054862843No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG960.4352754477442757No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG950.43074132849693947No Hit
GCAAAAGTTCTCAGGGATGCAGCAACTCTGCGTTGATACCACTGCTTCCC950.43074132849693947Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCACCCCATGTA920.41713897075493084No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA850.3854001360235774Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG830.376331897528905No Hit
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCACTGCTTCCC690.3128542280661981Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA690.3128542280661981No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT600.2720471548401723No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA600.2720471548401723No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC570.2584447970981637No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC550.24937655860349126Clontech SMART CDS Primer II A (95% over 24bp)
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG530.24030832010881886No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT500.22670596236681026No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC470.21310360462480166No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA420.1904330083881206No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA410.1858988891407844No Hit
GCAAAAGTTCTCAGGGATGCAGCAACTCTGCGTTGATACCACCCCATGTA410.1858988891407844No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC390.17683065064611198No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG380.1722965313987758No Hit
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCCCATGTACTC370.16776241215143958No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC360.1632282929041034No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA340.15416005440943098No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG320.14509181591475856No Hit
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCACTGCTTCCA320.14509181591475856Clontech Universal Primer Mix Long (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG300.13602357742008614No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA270.12242121967807754No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA260.11788710043074133Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTTACGCAG250.11335298118340513No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.11335298118340513No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.11335298118340513No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC250.11335298118340513No Hit
GCAAAAGTTCTCAGGGATGTCCAATGCCTGCGACAGATTTCATGTAGAGG240.10881886193606892No Hit
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCACCCCATGTA230.10428474268873271No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)