FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28849
Total Bases4.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA883830.635377309438798No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA522718.118478976740963No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA355112.308918853339804No Hit
GCAAAAGTTCTCAGGGATGTCCAACCCTGTGATGATTGATGCTTACGCAG28029.712641686020312No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC5071.75742660057541No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA4401.5251828486256023No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG3191.1057575652535616No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG3021.046830046102118No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1700.5892751915144373No Hit
GCAAAAGTTCTCAGGGATGTGCAACTCTGCGTTGATACCACTGCTTCCCA1580.5476792956428299Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC1280.4436895559638116No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG1190.41249263406010606No Hit
GCAAAAGTTCTCAGGGATGTGCAACCCTGTGATGATTGATGCTTACGCAG1190.41249263406010606No Hit
GTGCTGTCTCTCTTTGATGGAGGGTGTGTGTGTGATTGTGTGTGTGTGTG1110.38476203681236787No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCGGGGCTCCTGGTGCTGA1090.37782938750043327No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA760.2634406738535131No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA670.23224375194980762No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA640.2218447779819058No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA540.187181531422233No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG530.18371520676626574No Hit
GCAAAAGTTCTCAGGGATGTCCAACTCTGCGTTGATACCACTGCTTCCCA530.18371520676626574Clontech Universal Primer Mix Long (96% over 26bp)
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG530.18371520676626574No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA500.1733162327983639No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG490.16984990814239662No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTTACGCAG490.16984990814239662No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG490.16984990814239662No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA470.16291725883046207No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG470.16291725883046207No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA470.16291725883046207No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC470.16291725883046207No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC450.1559846095185275No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA440.15251828486256022No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC440.15251828486256022No Hit
TGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC430.14905196020659295No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGTTACGC370.12825401227078928No Hit
GTGCTGTCTCTCTTTGATGGAATCTCCATCCGGTCCCAGGGCAGACTCCC300.10398973967901833No Hit
GCAAAAGTTTCAGGGATGTCCAACCCTGTGATGATTGATGCTTACGCAGA300.10398973967901833No Hit
GTGCTGTCTCTCTTTGATGGAATCACTACAGGGCTCCTGGTGCTGAAGGA300.10398973967901833No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCCTCCTT290.10052341502305105No Hit
GCAAAGTTCTCAGGGATGTCCAACCCTGTGATGATTGATGCTTACGCAGA290.10052341502305105No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC290.10052341502305105No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)