FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12123
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length136-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT743261.30495751876598No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA213617.619401138332098No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1951.6085127443702056No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1681.3857955951497154No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA920.7588880640105584Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC630.5196733481811433No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA510.4206879485275922No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA470.3876928153097418No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA450.3711952487008166Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT390.32170254887404104No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA350.2887074156561907No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT340.28045863235172813No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA340.28045863235172813No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA300.24746349913387775No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT270.22271714922048996No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT250.20621958261156478No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA250.20621958261156478No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT240.1979707993071022No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT240.1979707993071022No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT220.181473232698177No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.181473232698177No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT210.17322444939371442No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC190.15672688278478925Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA190.15672688278478925No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT180.14847809948032664No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCGCTTACTCT180.14847809948032664No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT180.14847809948032664No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCC170.14022931617586407No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT170.14022931617586407No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT160.13198053287140146No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT160.13198053287140146No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT160.13198053287140146No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA150.12373174956693887No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT150.12373174956693887No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC150.12373174956693887No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT150.12373174956693887No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT150.12373174956693887No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA150.12373174956693887No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT140.11548296626247628No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC140.11548296626247628Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA140.11548296626247628No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT130.1072341829580137No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA130.1072341829580137Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTGAAAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA130.1072341829580137No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA130.1072341829580137No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)