FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184
Total Bases606.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA223153.32217973231358No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA80819.311663479923517No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA1393.3221797323135758No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC671.601338432122371No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT471.1233269598470363Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC461.0994263862332696No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA340.8126195028680689No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA290.6931166347992351No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG220.5258126195028681No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG220.5258126195028681No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.5019120458891013No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC210.5019120458891013No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT180.43021032504780116No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT150.35850860420650094Clontech Universal Primer Mix Long (96% over 26bp)
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG150.35850860420650094No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC130.3107074569789675No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG110.26290630975143403No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG100.23900573613766732No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC100.23900573613766732No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG90.21510516252390058No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC90.21510516252390058Clontech SMART CDS Primer II A (95% over 24bp)
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG80.19120458891013384No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA80.19120458891013384No Hit
GCAAAAGTTCTCAGGGATGTCCTGGTCTACAGGAACTAGAGGACTATTTA70.16730401529636713No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCGGAAGCAGCA70.16730401529636713No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT70.16730401529636713No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT60.14340344168260039Clontech SMART CDS Primer II A (96% over 25bp)
GTGCTGTCTCTTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC60.14340344168260039No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAGGCAGCA60.14340344168260039No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC60.14340344168260039No Hit
GTGCTGTCTCCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC60.14340344168260039No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAACAGCA50.11950286806883366No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA50.11950286806883366No Hit
GGTGTTTGGGATGGAATGGCGATCCACGCAGGTGGTTCGCAGAAGCAGCA50.11950286806883366No Hit
GTGCTGTCTCTCTTTGAGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC50.11950286806883366No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG50.11950286806883366No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG50.11950286806883366No Hit
GGTGTTTGGGATGGAATGGTGACCCACGCAGGTGGTTCGCAGAAGCAGCA50.11950286806883366No Hit
TGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC50.11950286806883366No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGCGGTTCGCAGAAGCAGCA50.11950286806883366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)