FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013
Total Bases436.3 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC39413.076667772983738No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC2407.965482907401261No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA1896.272817789578493No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1615.343511450381679Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT1093.6176568204447395Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG1053.484898771988052No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT892.9538665781613007No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC882.920677066047129Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG782.58878194490541No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT652.157318287421175No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC571.8918021905077995No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC290.9624958513109858No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG290.9624958513109858No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA220.7301692665117823Clontech SMART CDS Primer II A (96% over 26bp)
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA210.6969797543976104No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG200.6637902422834384No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA200.6637902422834384Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA190.6306007301692665No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCATGT190.6306007301692665No Hit
GCAAAAGTTCTCAGGGATGTGTCATCCCCAAGCAGCGGTCATCCCCAAGC180.5974112180550946No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAT170.5642217059409227No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT160.5310321938267508No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA150.49784268171257884No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT130.431463657484235Clontech SMART CDS Primer II A (96% over 25bp)
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC130.431463657484235No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA120.39827414537006306No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA110.36508463325589113No Hit
GTGCTGTCTCTCTTTGATGGACTCTGCGTTGATACCACTGCTTCCCATGT110.36508463325589113Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA110.36508463325589113No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC110.36508463325589113No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCCTCCTT110.36508463325589113No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA100.3318951211417192No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA100.3318951211417192No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC90.2987056090275473No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTTACGCAG80.2655160969133754No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCCATGTACTCTGCGTTGATA70.23232658479920343No Hit
GTGCTGTCTCTCTTTGATGGACGGCACCCCATGTACTCTGCGTTGATACC70.23232658479920343No Hit
CTCCAAGTAACGGTGCTGAGAAAGCAGAAATGAGTTCAGCAAAGTGAGGA70.23232658479920343No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTACTCTGCGTTGAT70.23232658479920343No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC70.23232658479920343No Hit
GCAAAAGTTCTCAGGGATGTCCAGGCTTTTTCATGGTTTAGTTCCTCACA70.23232658479920343No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG70.23232658479920343No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGTTACGC60.19913707268503153No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCCTCC60.19913707268503153No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG60.19913707268503153No Hit
CTCCAAGTAACGGTGCTTTCTCTTCACCCTTTGCCCTTACGCAGACCAAG60.19913707268503153No Hit
GTGCTGTCTCTCTTTGATGGACAGCCCCCCCATGTACTCTGCGTTGATAC60.19913707268503153No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCCCTG60.19913707268503153Clontech Universal Primer Mix Long (96% over 27bp)
CAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATACC60.19913707268503153No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCCCAT60.19913707268503153No Hit
GCAAAAGTTCTCAGGGATGTCCCCCCATGTACTCTGCGTTGATACCACTG50.1659475605708596Clontech SMART CDS Primer II A (95% over 23bp)
GCAAAAGTTCTCAGGGATGTCCCATGTACTCTGCGTTGATACCACTGCTT50.1659475605708596Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCGGAC50.1659475605708596No Hit
CTCCAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTGC50.1659475605708596No Hit
GCAAAAGTTCTCAGGGATGTGTCATCCCCAAGCAGCTGGTCATCCCCAAG50.1659475605708596No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGAGACCCCCA50.1659475605708596No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC50.1659475605708596No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCCATG50.1659475605708596No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTA50.1659475605708596No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGGCTCTC40.1327580484566877No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACCCCATGTACTCT40.1327580484566877Clontech SMART CDS Primer II A (95% over 21bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCGC40.1327580484566877No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTGGTTGTTACGCAGAC40.1327580484566877No Hit
GCAAAAGTTCTCAGGGATGTGAGGCCCACCCATGTACTCTGCGTTGATAC40.1327580484566877No Hit
GTGCTGTCTCTCTTTGATGGAGCGGTGCCTCCTTCTCTTTTGGTTGTTAC40.1327580484566877No Hit
GTGCTGTCTCTCTTTGATGGACAAGTCACCCAAGCAGGTTGACATCAGGT40.1327580484566877No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTATC40.1327580484566877No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATCCCCCAT40.1327580484566877No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCCCTG40.1327580484566877Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTTAGGGAGACCC40.1327580484566877No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTCTCTTTTGGTTGTTACGCAGAC40.1327580484566877No Hit
GCAAAAGTTCTCAGGGATGTGATGATTGATGCTTACGCAGACCAAGTCTC40.1327580484566877No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)