FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013
Total Bases436.1 kbp
Sequences flagged as poor quality0
Sequence length139-145
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA74024.560238964487223Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA35211.682708264188516No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1976.538333886491869Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT1846.106870229007633No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1183.9163624294722865Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1073.5512777962163957No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC782.58878194490541No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA642.1241287753070033Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC632.090939263192831Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC632.090939263192831Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA361.1948224361101891No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA290.9624958513109858No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC270.8961168270826418No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA220.7301692665117823No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC200.6637902422834384No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA200.6637902422834384No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCTCTAT190.6306007301692665No Hit
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA170.5642217059409227No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT160.5310321938267508No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT120.39827414537006306No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGCTGC110.36508463325589113No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA110.36508463325589113No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG100.3318951211417192No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT100.3318951211417192No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG90.2987056090275473No Hit
GCATCAATCATCACAGGGTTCATGGGAAGCAGTGGTATCAACGCAGAGTA80.2655160969133754Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA80.2655160969133754No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCTCTATG80.2655160969133754No Hit
GCATCATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC80.2655160969133754Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGGTACATGGGGAAGCAGTGGTATCAACGCAGAGT80.2655160969133754Clontech Universal Primer Mix Long (100% over 23bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGTGGT70.23232658479920343Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC70.23232658479920343Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAAGAGAAAGCACCGTTACTTGGAGCCTCTATGTAGAAC60.19913707268503153No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCTCTA60.19913707268503153No Hit
CAACCAAAAGAGAAGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGGT50.1659475605708596Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA50.1659475605708596Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA50.1659475605708596No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGCTGCT50.1659475605708596No Hit
GCTCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC50.1659475605708596Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACATCCCTGAGAACTTTTGCCCTCTATGTAGAACCATG40.1327580484566877No Hit
CAACCAAAAGAAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG40.1327580484566877No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATCAAAG40.1327580484566877No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG40.1327580484566877Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC40.1327580484566877Clontech Universal Primer Mix Long (96% over 26bp)
GCATAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC40.1327580484566877Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA40.1327580484566877No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGGA40.1327580484566877Clontech Universal Primer Mix Long (96% over 25bp)
TCCTATCAGTGGCAGCACCGTTACTGGAGCCTCTATGTAGAACCATGTCG40.1327580484566877No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATAA40.1327580484566877No Hit
CAACCAAAAGAGAAGGAGCACCATCAAAGAGAGACAGCACCATCAAAGAG40.1327580484566877No Hit
CAACCAAAAGAGAAGGAGGCACCCTCCATCAAAGAGAGACAGCACCCTCC40.1327580484566877No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT40.1327580484566877No Hit
CAACCAAAAGAGAAGGAGGCACCGCTCCATCAAAGAGAGACAGCACCCTC40.1327580484566877No Hit
GCATCAATCATCACAGGGTACATGGAAAGCAGTGGTATCAACGCAGAGTA40.1327580484566877Clontech SMARTer II A Oligonucleotide (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)