FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18789
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT679636.17009952631859No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA462824.631433285432966No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA16949.015913566448454No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT3191.6978019053701634No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2751.463622332215658Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACTCATCGTCCCTCACAGCAGCCGCCGCCG2541.3518548086646442No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1850.9846186598541701No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1740.9260737665655436No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA1490.7930171909095748No Hit
GGGCAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA1090.5801266698600245No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1060.5641598807813082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT900.4790036723614881No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC890.4736814093352493No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA860.4577146202565331No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT700.37255841183671295No Hit
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT640.3406248336792804No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA560.29804672946937033No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA510.2714354143381766No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA480.25546862525946035Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA470.25014636223322156No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT470.25014636223322156No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA440.2341795731545053No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA410.21821278407578903No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA410.21821278407578903No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT390.20756825802331152No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGTTGGACTGTAGAAC360.19160146894459523No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC360.19160146894459523Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA350.18627920591835648No Hit
CAACCAAAAGAGAAGGAGGCACCGTCCATCAAAGAGAGACAGCACCGTCC330.17563467986587897No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCTTGGAC330.17563467986587897No Hit
GCATCAATCATCACAGGGTTGGGCGGGTCTTCTTCCTTCTCGCCTAACGC300.1596678907871627No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT300.1596678907871627No Hit
CAACCAAAAGAGAAGGAGGCCCCATCAAAGAGAGACAGCACCATCAAAGA300.1596678907871627No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT290.15434562776092395No Hit
CAACCAAAAGAGAAGGAGGCACGTCCATCAAAGAGAGACAGCACTTGGAC280.14902336473468517No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.1437011017084464No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG260.13837883868220768No Hit
GGGCAAAGGGTGAAGGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA240.12773431262973017No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG240.12773431262973017No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA230.1224120496034914No Hit
TCCTATCAGTGGCCGCAAAGAAGACGAAAAAGTCGCTGGAGTCGATCAAC230.1224120496034914No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG230.1224120496034914No Hit
CAACCAAAAGAGAAGGAGGCACGGGTGTTCTGTGCCAAGTGCTCCCAAGT220.11708978657725265No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT200.10644526052477513No Hit
CAACCAAAAGAGAGACAGCACCATCAAAGAGAGACAGCACCATCAAAGAG200.10644526052477513No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT200.10644526052477513No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGATCAAAGA200.10644526052477513No Hit
AACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG200.10644526052477513No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT190.10112299749853637No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA190.10112299749853637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)