FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18679
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA536028.695326302264572No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA461624.71224369612934No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC8634.620161678890733No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC4542.4305369666470367No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG2831.5150703999143422No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA2601.3919374698859681No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1770.9475882006531399No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT1710.9154665667326944No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1630.872637721505434No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA1600.8565769045452112No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1360.7280903688634296No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1350.722736763210022Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAGCTTCCTGAGATTAAACATAAAGAAGAAA1020.5460677766475721No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT920.4925317201134965No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA900.4818245088066813No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG900.4818245088066813No Hit
GTGCTGTCTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAG810.4336420579260132No Hit
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA800.4282884522726056No Hit
GTGCTGTCTCTCTTTGATGGGCTAAAGACCAAGTCTCTGCTACCGTAGCA800.4282884522726056No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA780.41758124096579047No Hit
GTGCTGTCTCTCTTTGATGGACCACCTCTTTGGGGCCCTGGTCCTCCTGC700.3747523957385299No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC650.34798436747149203No Hit
GTGCTGTCTCTCTTTGATGGACGAGGGGTTCCTCCTCCTCCTCAATTGCT630.3372771561646769No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT610.3265699448578618Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA550.29444831093741636No Hit
GCAAAAGTTCTCAGGGATGTCCGGTTGTTTTAAAGTCTGTAGCCCCTCCC540.28909470528400877No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC510.27303388832378606Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC500.2676802826703785No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTACAGGGCTCCTGGTGCTGA500.2676802826703785No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC470.2516194657101558No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC470.2516194657101558No Hit
GTGCTGTCTCTCTTTGATGGAATCCTCACAGGATCTAGTTGTTTAAAAGT460.24626586005674825No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCGGCTAAAGACCAAG430.2302050430965255No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT420.22485143744311792No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGAGGCTAAA420.22485143744311792No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA420.22485143744311792No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA350.18737619786926496No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTATC330.1766689865624498No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA320.17131538090904222No Hit
GCAAAAGTTCTCAGGGATGTCGTCATCGTGGAGCGTGGGAAGGGCGACGG320.17131538090904222No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG310.16596177525563466No Hit
GCAAAAGTTCTCAGGGATGTCCTCAGGTTGTGCCTCCTTCTCTTTTGGTT310.16596177525563466No Hit
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA310.16596177525563466No Hit
GTGCTGTCTCTCTTTGATGGATGGGAGCCCTGGGGCACCCAGGCCGACCT310.16596177525563466No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTA270.14454735264200438No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA260.13919374698859682No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC260.13919374698859682No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA250.13384014133518926No Hit
GTGCTGTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAGCA240.1284865356817817No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGGGCTAA240.1284865356817817No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA240.1284865356817817No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA220.11777932437496653No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA220.11777932437496653No Hit
GTGCTGTCTCTCTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAGC210.11242571872155896No Hit
CTCCAAGTAACGGTGCTGTCTGTTGGCTGGTGCAGGGCTCCTGGTGCTGA210.11242571872155896No Hit
GTGCTGTCTCTCTTTGATGGAATCCCTCTTCCCTGTTACATCTCCTTTGT210.11242571872155896No Hit
GTGCTGTCTCTCTTTGATGGAGGGTGCTGTCTCTCTTTGATGGAGGGTGC210.11242571872155896No Hit
GCAAAAGTTCTCAGGGATGTCTGGTTGTTTTAAAGTCTGTAGCCCCTCCC210.11242571872155896No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC210.11242571872155896No Hit
GTGCTGTCTCTCTTTGATGGAGATAGCAGGGGAGTAGGGAACCCCAGAGG200.1070721130681514No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGTAGGGCTCCTGGTGCTGA190.10171850741474382No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)