FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35882
Total Bases5.1 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1235734.43787971684967No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA485213.522100217379188No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA24216.747115545398808No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC13203.678724708767627No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC13203.678724708767627No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT9142.5472381695557664No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT6891.9201828214703751No Hit
GTGCTGTCTCTCTTTGATGGAATCCATGCCTGAATCTGCGGTATACAATT5821.6219831670475446No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT5121.4268992809765342Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA4291.195585530349479No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA2850.7942701075748286No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC2820.7859093696003566No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT2740.7636140683350984No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT2340.6521375620088066Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC2230.6214815227690764Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1690.4709882392285826No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1610.4486929379633242No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1490.41524998606543667No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG1490.41524998606543667No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1230.3427902569533471No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC1140.31770804302993144No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA1010.2814781784738867No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC1010.2814781784738867No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG930.2591828772086283No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGAGGCTAAA880.2452483139178418No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC870.24246140125968452No Hit
GCAAAAGTTCTCAGGGATGTTAGAGCATAGCAACTCTGCGTTGATACCAC840.23410066328521262No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG780.21737918733626885No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC760.2118053620199543No Hit
GTGCTGTCTCTCTTTGATGGAGGTTAGAGCATAGCAACTCTGCGTTGATA680.18951006075469595No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT670.18672314809653864No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG660.18393623543838136No Hit
GTGCTGTCTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAT650.18114932278022405No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG600.16721475948943762No Hit
GTGCTGTCTCTCTTTGATGGAGAGTGAGTTATCACAAGATGTGTTTGTTT600.16721475948943762No Hit
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA580.16164093417312303No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG500.13934563290786467No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA460.1281979822752355No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG460.1281979822752355No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA440.1226241569589209No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG440.1226241569589209No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA430.1198372443007636No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG400.11147650632629172No Hit
GTGCTGTCTCTCTTTGATGGGCTAAAGACCAAGTCTCTGCTACCGTATGA400.11147650632629172No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC390.10868959366813442No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT380.10590268100997716Clontech SMART CDS Primer II A (96% over 25bp)
GTGCTGTCTCTCTTTGATGGACTCTGCGTTGATACCACTGCTTCCCATGT360.10032885569366257Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA360.10032885569366257Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)