FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12465
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA510040.91456077015644No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT165313.261131167268351No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA11148.937023666265544No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA5074.067388688327316No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTTGGACTGT1981.5884476534296028No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1230.9867629362214199No Hit
GGGCAAAGGGTGAAGAGAAAGTGGGCAAATGAACAAAATGAAAGGAGGCA1200.9626955475330927No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA1080.8664259927797834No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC1020.8182912154031288No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC940.7541115122342559No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGT740.5936622543120739No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA620.49739269955876453No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA590.4733253108704373Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGACAGCACCGTTACTTGGAGTTGGACTGTAGAACCATGTC580.46530284797432814No Hit
CAACCAAAAGAGAAGGAGGCACTTGGACTGTAGAACCATGTCGTCAGTGT570.457280385078219No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA560.44925792218210986No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA560.44925792218210986No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC450.36101083032490977No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA420.3369434416365824No Hit
CAACCAAAAGAGAAGGAGGCACAAACTGCTGAGTAACCTGCTGTGCAACC380.304853590052146No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCTTGGAC370.29683112715603693No Hit
CAACCAAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTA360.2888086642599278No Hit
CATCAAAGAGAGACAGCACTTGGACTGTAGAACCATGTCGTCAGTGTAGA360.2888086642599278No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT270.21660649819494585No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGTTGGACTGTAGAAC260.20858403529883676No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA260.20858403529883676No Hit
GGGCAAAGGGTGAAGGGCAAATGAACAAAATGAAAGGAGGCAAGGGCTGC250.20056157240272765No Hit
CAACCAAAAGAGAAGGAGGCACTGAACCCCAGTGGGGAGACCAGAAGTGC250.20056157240272765No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA240.1925391095066185No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA240.1925391095066185No Hit
CAACCAAAAGAGAAGGAGGCACTATTAATAATCTTGTCACAGCAAAAGAT230.18451664661050943No Hit
CAACCAAAAGAGAAGGAGGCACGGAGAATGATCTCCGCATCCTGGAGCAG230.18451664661050943No Hit
CAACCAAAAGAGAAGGAGGCACCGAGCCTGGTGCAGTGGTATCAACGCAG220.17649418371440032No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGTTGGACT200.1604492579221821No Hit
CAACCAAAAGAGAAGGAGGCACCACACTACTTGACTTCAAACTATACTAC190.152426795026073No Hit
CAACCAAAAGAGAAGGAGGCACACCCTGGGTCGTTTCTGTCCGTGTGCTT190.152426795026073No Hit
GCATCAATCATCACAGGGTTGGACTTCTGCGCAGCTCCTGATCACTTTGT190.152426795026073No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG190.152426795026073No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA170.13638186923385479No Hit
CAACCAAAAGAGAAGGAGGCACTGGCAGAGGTGCCATAGAAGCAGTGAAC170.13638186923385479No Hit
CAACCAAAAGAGAAGGAGGCCCCATCAAAGAGAGACAGCACCATCAAAGA170.13638186923385479No Hit
CAACCAAAAGAGAAGGAGGCACCAGAGAAAGGTGAAGTCCAATTTAACGA160.12835940633774567No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAGCAAAATGAAAGGAGGCA160.12835940633774567No Hit
GGGCAAAGGGTGAAAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA160.12835940633774567No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA140.11231448054552746No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGACA130.10429201764941838No Hit
CAACCAAAAGAGAAGGAGGCACTGCCTCCACTACAGCAACTGCACCCACG130.10429201764941838No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA130.10429201764941838Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACTGAGAGGAGGGGAGGGATGGTAGAGTTT130.10429201764941838No Hit
GGGCAAAGGGTGAGGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA130.10429201764941838No Hit
GGGCAAAGGGTGAAGAGAAAGAGGACAAATGAACAAAATGAAAGGAGGCA130.10429201764941838No Hit
GCATCAATCATCACAGGGTTGGTTCGGCTGATCTGGCTGGCTAGGCGGGT130.10429201764941838No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG130.10429201764941838No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)