FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30421
Total Bases4.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA865428.447454061339204No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA422713.895006738765984No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT360811.860228131882582No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA24157.938595049472403No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC14494.763157029683442No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGAATCAAGCAA4811.5811446040564083No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC3951.2984451530192958No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG3391.1143617895532691No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGGGCTAAAGA3381.1110745866342329No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT2780.9138424114920614No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG2000.6574405838072385No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGAGGCTAAAGACCAAGTCTCTG1560.512803655369646No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1510.496367640774465Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGATTGGGTTAATTCAAAAGCCTTGTCTTCAA1300.427336379474705No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT1180.3878899444462707No Hit
GTGCTGTCTCTCTTTGATGGAATGGGTTAATTCAAAAGCCTTGTCTTCAA1100.36159232109398115No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT1050.3451563064988002Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGACCGGTAGAGGCTAAAAGAAAGGCCCAGGT1010.3320074948226554No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG840.27612504519904013No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG760.24982742184675058No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA750.2465402189277144No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC740.24325301600867819Clontech SMART CDS Primer II A (95% over 24bp)
GTGCCTCCTTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTA730.23996581308964202No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGAGGCTAAAGACCAAGTCTCTG700.23010420433253342No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA690.22681700141349725No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC690.22681700141349725No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT660.21695539265638866No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA630.20709378389928013No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG550.18079616054699058No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA480.15778574011373722No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG480.15778574011373722No Hit
GTGCTGTCTCTCTTTGATGGAGCCGTTCTGCTCTGTGTTGTCCACTCCAG460.15121133427566485No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG460.15121133427566485No Hit
GTGCTGTCTCTCTTTGATGGGCTAAAGACCAAGTCTCTGCTACCGTAGCT460.15121133427566485No Hit
GTGCTGTCTCTCTTTTGGTTGGGCTAAAGACCAAGTCTCTGCTACCGTAG440.14463692843759246No Hit
GTGCTGTCTCTCTTTGATGGAGGAGGTGGAGGAGGAGGAGGAGGAGAAGA410.1347753196804839No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC410.1347753196804839No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG400.1314881167614477No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCGGCTAAAGACCAAGTCTCT380.12491371092337529No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGAGGCTAAA350.11505210216626671No Hit
GTGCTGTCTCTCTTTGATGGAATCGTTGGTTCCCTCTCAAACCAGAGCAG350.11505210216626671No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTATC330.10847769632819433No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTTAGGAATCAAG330.10847769632819433No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)